IMMUNE-RESPONSE GENE-FUNCTION CORRELATES WITH THE EXPRESSION OF AN IA-ANTIGEN .1. PREFERENTIAL ASSOCIATION OF CERTAIN A-EPSILON AND E-ALPHA CHAINS RESULTS IN A QUANTITATIVE DEFICIENCY IN EXPRESSION OF AN A-EPSILON-E-ALPHA COMPLEX
IMMUNE-RESPONSE GENE-FUNCTION CORRELATES WITH THE EXPRESSION OF AN IA-ANTIGEN .1. PREFERENTIAL ASSOCIATION OF CERTAIN A-EPSILON AND E-ALPHA CHAINS RESULTS IN A QUANTITATIVE DEFICIENCY IN EXPRESSION OF AN A-EPSILON-E-ALPHA COMPLEX
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DOI：
10.1084/jem.155.2.490
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发表时间：
1982-01-01
影响因子：
15.3
通讯作者：
JONES, PP
中科院分区：
文献类型：
--
作者：
MCNICHOLAS, JM;MURPHY, DB;JONES, PP
I-Eu不能与I-Ak或I-As在F1小鼠中相互作用，以允许对抗原鸽子细胞色素c的反应，不像I-E亚区来自其他Ia.7+ halotypes。进行血清学和生物化学分析以确定来自这些F1小鼠的细胞是否表达。图形 **。复合物，应作为限制性元件的T细胞识别鸽细胞色素c的抗原呈递细胞。Y-17单克隆抗体识别某些Ae：E α上的组合或构象决定簇Ia.m44。复合物，用于区分。**图形 **。和 **图形 **。细胞表面的复合物。虽然Y-17的补体依赖性微细胞毒性未能检测到。图形 **。复合物的细胞从适当的F1小鼠，这些分子被检测到定量吸收和定量免疫荧光研究。.**图形 **。发现复合物仅以纯合I-Ab、I-Ek、I-Ak、I-Ek和I-As、I-Ek细胞表达水平的1/8-1/7的水平存在。双向聚丙烯酰胺凝胶电泳分析结果表明，低水平的表达。图形 **。复合物是优先缔合的结果。**图形 **。和 **图形 **。在F1细胞中相互连接。
I-Eu fails to interact with I-Ak or I-As in F1 mice to allow a response to the antigen pigeon cytochrome c, unlike I-E subregions derived from other Ia.7+ halotypes. Serological and biochemical analyses were performed to determine whether cells from these F1 mice express the .**GRAPHIC**. complexes that should function as restriction elements for T cell recognition of pigeon cytochrome c on antigen-presenting cells. The Y-17 monoclonal antibody, which recognizes the combinatorial or conformational determinant Ia.m44 on certain Ae:E.alpha. complexes, was used to distinguish between .**GRAPHIC**. and .**GRAPHIC**. complexes on cell surfaces. Although complement-dependent microcytotoxicity with Y-17 failed to detect .**GRAPHIC**. complexes on cells from appropriate F1 mice, these molecules were detected by quantitative absorption and quantitative immunofluorescence studies. .**GRAPHIC**. complexes were found to be present at levels only 1/8-1/7 the levels expressed by homozygous I-Ab, I-Ek; I-Ak, I-Ek; and I-As, I-Ek cells. The results of 2-dimensional polyacrylamide gel electrophoresis analyses suggest that the low levels of expression of .**GRAPHIC**. complexes are a consequence of the preferential association of .**GRAPHIC**. and .**GRAPHIC**. chains with each other in the F1 cells.