INHIBITION OF G1 CYCLIN EXPRESSION AND G1 CYCLIN-DEPENDENT PROTEIN-KINASES BY CAMP IN AN ASTROCYTIC CELL-LINE
INHIBITION OF G1 CYCLIN EXPRESSION AND G1 CYCLIN-DEPENDENT PROTEIN-KINASES BY CAMP IN AN ASTROCYTIC CELL-LINE
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DOI：
10.1006/bbrc.1994.2753
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
TORUDELBAUFFE, D
中科院分区：
文献类型：
--
作者：
GAGELIN, C;PIERRE, M;TORUDELBAUFFE, D
使用星形胶质细胞系检查cAMP对细胞周期进程的影响。我们发现，forskolin和8-bromo-cAMP阻断碱性成纤维细胞生长因子诱导的DNA合成，不抑制丝裂原活化蛋白激酶的活化，但它们降低G1期细胞周期蛋白（E和D1）的表达，而不改变细胞周期蛋白A的水平，此外，它们抑制细胞周期蛋白A和细胞周期蛋白E依赖的组蛋白H1激酶的活化。这些结果表明，cAMP可能通过调节细胞周期蛋白的合成和细胞周期蛋白依赖性激酶的激活发挥其抗增殖作用。(C)1994年出版社出版。
The effects of cAMP on cell cycle progression were examined using an astrocytic cell line. We show that forskolin and 8-bromo-cAMP block the basic Fibroblast Growth Factor-induced DNA synthesis, do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin (E and D1) expression without modification of cyclin A level, Furthermore, they inhibit the activation of cyclin A- and cyclin E-dependent histone H1 kinases. These results suggest that cAMP may exert its antiproliferative effects through the regulation of cyclin synthesis and cyclin-dependent kinase activation. (C) 1994 Academic Press, Inc.