Analysis of Lung Microbiota in Bronchoalveolar Lavage, Protected Brush and Sputum Samples from Subjects with Mild-To-Moderate Cystic Fibrosis Lung Disease.
Analysis of Lung Microbiota in Bronchoalveolar Lavage, Protected Brush and Sputum Samples from Subjects with Mild-To-Moderate Cystic Fibrosis Lung Disease.
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DOI：
10.1371/journal.pone.0149998
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ashare A
中科院分区：
文献类型：
--
作者：
Hogan DA;Willger SD;Dolben EL;Hampton TH;Stanton BA;Morrison HG;Sogin ML;Czum J;Ashare A
患有囊性纤维化（CF）的个体经常获得慢性肺部感染，导致不可逆的损伤。我们试图检查轻中度CF肺病患者肺部微生物群落的区域变化，检查局部微生物群与局部损伤之间的关系，并确定直接从肺部采集的样本中的微生物群与自发咳痰中的微生物群之间的关系。在这项初步研究中，9名稳定的成年CF患者（FEV1>50%）通过支气管肺泡灌洗（BAL）和粘液栓保护刷（PB）采样对右肺不同肺叶进行区域采样。在手术前即刻从9名受试者中的6名获得痰液样本。对从这些样本中提取的DNA进行微生物群落分析，并从最近的CT扫描中量化每个肺叶的损伤程度。在右肺区域观察到的损伤程度与特定微生物属、群落多样性或组成水平或每毫升支气管肺泡灌洗液中的细菌基因组拷贝数无关。在所有受试者中，来自肺不同区域的BAL液含有相似的微生物群落。在9名受试者中的8名中，来自肺的不同区域的PB样本的微生物群落组成也相似，并且与来自同一肺叶的BAL液中的微生物群落相似。PB样品中的微生物群落比BAL样品中的微生物群落更多样化，这表明在粘液塞中富集了一些类群。据我们所知，这项研究是第一个检查临床稳定的轻度至中度CF相关肺病患者CF肺不同区域微生物群的研究。
Individuals with cystic fibrosis (CF) often acquire chronic lung infections that lead to irreversible damage. We sought to examine regional variation in the microbial communities in the lungs of individuals with mild-to-moderate CF lung disease, to examine the relationship between the local microbiota and local damage, and to determine the relationships between microbiota in samples taken directly from the lung and the microbiota in spontaneously expectorated sputum. In this initial study, nine stable, adult CF patients with an FEV1>50% underwent regional sampling of different lobes of the right lung by bronchoalveolar lavage (BAL) and protected brush (PB) sampling of mucus plugs. Sputum samples were obtained from six of the nine subjects immediately prior to the procedure. Microbial community analysis was performed on DNA extracted from these samples and the extent of damage in each lobe was quantified from a recent CT scan. The extent of damage observed in regions of the right lung did not correlate with specific microbial genera, levels of community diversity or composition, or bacterial genome copies per ml of BAL fluid. In all subjects, BAL fluid from different regions of the lung contained similar microbial communities. In eight out of nine subjects, PB samples from different regions of the lung were also similar in microbial community composition, and were similar to microbial communities in BAL fluid from the same lobe. Microbial communities in PB samples were more diverse than those in BAL samples, suggesting enrichment of some taxa in mucus plugs. To our knowledge, this study is the first to examine the microbiota in different regions of the CF lung in clinically stable individuals with mild-to-moderate CF-related lung disease.