Sonic hedgehog signaling is important in tooth root development
Sonic hedgehog signaling is important in tooth root development
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DOI：
10.1177/154405910608500506
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发表时间：
2006-05-01
影响因子：
7.6
通讯作者：
Ota, MS
中科院分区：
文献类型：
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作者：
Nakatomi, M;Morita, I;Ota, MS
Hertwig‘s上皮根鞘(HERS)对牙根的形成很重要，但调控牙根发育的分子基础尚不清楚。我们假设Sonic hedgehog(Shh)信号参与了HERS的功能，因为它在胚胎牙齿发育过程中介导了上皮-间充质的相互作用。我们研究了Shh信号在小鼠磨牙根发育中的基因表达模式。Shh和Patched2转录本在Her中表达，而Patched1、Smoothens和Gli1除了在Her中表达外，还在增生性牙间充质中表达。为了证实Shh信号在体内是否具有生理功能，我们分析了携带Patched1蛋白C末端异常的间充质发育不良(MES)小鼠。在突变体中，细胞增殖在1wk时受到抑制。此外，磨牙萌出受到干扰，在4周时所有的根都比对照短。这些结果表明Shh信号在牙根发育过程中起着重要作用。使用的缩写：BrdU，5-溴-2‘-脱氧尿苷；HERS，Hertwig’s上皮根鞘；NFI-C/CTF，核因子I-c/CAAT盒转录因子；PCNA，增殖细胞核抗原；PTC，补丁；Shh，声波刺猬；Smo，平滑。
Hertwig's epithelial root sheath ( HERS) is important for tooth root formation, but the molecular basis for the signaling of root development remains uncertain. We hypothesized that Sonic hedgehog (Shh) signaling is involved in the HERS function, because it mediates epithelial-mesenchymal interactions during embryonic odontogenesis. We examined the gene expression patterns of Shh signaling in murine developing molar roots. Shh and Patched2 transcripts were identified in the HERS, whereas Patched1, Smoothened, and Gli1 were expressed in the proliferative dental mesenchyme in addition to the HERS. To confirm whether Shh signaling physiologically functions in vivo, we analyzed mesenchymal dysplasia (mes) mice carrying an abnormal C-terminus of the PATCHED1 protein. In the mutant, cell proliferation was repressed around the HERS at 1 wk. Moreover, the molar eruption was disturbed, and all roots were shorter than those in control littermates at 4 wks. These results indicate that Shh signaling is important in tooth root development. Abbreviations used: BrdU, 5-bromo-2'-deoxyuridine; HERS, Hertwig's epithelial root sheath; NFI-C/CTF, nuclear factor I-c/ CAAT box transcription factor; PCNA, proliferating cell nuclear antigen; Ptc, patched; Shh, sonic hedgehog; Smo, smoothened.