Aquaporins and their function in root water transport under salt stress conditions in Eutrema salsugineum
Aquaporins and their function in root water transport under salt stress conditions in Eutrema salsugineum
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盐胁迫条件下水通道蛋白及其在根部水分运输中的功能
DOI：
10.1016/j.plantsci.2019.110199
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发表时间：
2019-10-01
期刊：
影响因子：
5.2
通讯作者：
Li, Guowei
中科院分区：
文献类型：
--
作者：
Qin, Shenghao;Liu, Yiyang;Li, Guowei
盐地黄皮（Eutrema salsugineum）是极端嗜热模式种。为了深入了解根导水率和水通道蛋白在E. salsugineum的，我们调查了水通道蛋白家族概况，植物水分状况和根导水率在标准（无盐）和盐胁迫条件下。我们发现，有没有变化时，盐浓度小于200 mM NaCl的叶片的相对电导率，和蒸腾速率下降到60.6%，在100 mM NaCl的14天相比，在标准条件下。压力室试验结果表明，E.盐胁迫抑制了salsugineum的生长。然而，通常用作水通道蛋白抑制剂的丙酸却意外地提高了E.盐柳碱E.分析了盐杉属植物PIP水通道蛋白的表达，并在转录和翻译水平上进行了研究。最后，两个EsPIP被确定在盐胁迫中发挥作用。本研究为盐生植物在盐胁迫条件下如何维持其水分状态和水通道蛋白的调控模式提供了证据。
Eutrema salsugineum is considered as extremophile model species. To gain insights into the root hydraulic conductivity and the role played by aquaporins in E. salsugineum, we investigated the aquaporin family profiles, plant water status and root hydraulic conductivity under standard (salt-free) and salt stress conditions. We found that there was no variation in the relative electric conductivity of the leaves when the salt concentration was less than 200 mM NaCl, and the transpiration rate dropped to 60.6% at 100 mM NaCl for 14 days compared to that at standard conditions. The pressure chamber techniques indicated that the root hydraulic conductivity of E. salsugineum was repressed by salt stress. However, propionic acid, usually used as an aquaporin inhibitor, unexpectedly enhanced the root hydraulic conductivity of E. salsugineum. The aquaporin family in E. salsugineum was profiled and the PIP aquaporin expression was investigated at the transcriptional and translational levels. Finally, two EsPIPs were identified to play a role in salt stress. The overall study provides evidence on how halophytes maintain their water status and aquaporin regulation pattern under salt stress conditions.