Human alveolar bone cells interact with ProRoot and tooth-colored MTA
Human alveolar bone cells interact with ProRoot and tooth-colored MTA
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DOI：
10.1016/j.joen.2006.03.019
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发表时间：
2006-09-01
影响因子：
4.2
通讯作者：
MacFarland, Don
中科院分区：
文献类型：
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作者：
Al-Rabeah, Ebtehal;Perinpanayagam, Hiran;MacFarland, Don
细胞对矿物三氧化物聚集体（MTA）的反应对于神经根周围组织的修复和再生是重要的。本研究的目的是分析人牙槽骨细胞对MTA的反应。从口腔外科手术中获得人牙槽骨芯片，并在补充有胎牛血清的最低必需培养基中生长3至4周后收获外植体培养物。将早期传代的细胞接种到预设的ProRoot（灰色）MTA、牙色（白色）MTA和用局部麻醉溶液制备的MTA上。扫描电镜显示细胞在24小时内贴附并铺展在MTA上，并在7天内增殖形成基质样层。细胞附着和细胞表面与灰色和白色MTA的相互作用，并与MTA与局部麻醉剂制备的增殖培养14天。手术来源的人牙槽骨细胞提供了一个临床相关模型，证明了ProRoot和牙齿颜色MTA支持细胞附着、增殖和基质形成的能力。
The cellular response to mineral trioxide aggregate (MTA) is important for the repair and regeneration of periradicular tissues. The purpose of this study was to analyze the response of human alveolar bone cells to MTA. A human alveolar bone chip was obtained from an oral surgical procedure and explant cultures harvested after 3 to 4 weeks of outgrowth in a-minimum essential medium supplemented with fetal calf serum. Cells in early passage were seeded onto preset ProRoot (gray) MTA, tooth-colored (white) MTA, and MTA prepared with local anesthetic solution. Scanning electron microscopy showed cells were attached and spread out onto MTA within 24 hours, and proliferated to form a matrix-like layer within 7 days. Cell attachment and cell-surface interactions with the gray and white MTA, and with the MTA prepared with local anesthetic were comparably propagated for 14 days. The surgically derived human alveolar bone cells provided a clinically relevant model that demonstrated the capacity of both ProRoot and tooth-colored MTA to support cell attachment, proliferation, and matrix formation.