Multicolor spectral karyotyping of human chromosomes
Multicolor spectral karyotyping of human chromosomes
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DOI：
10.1126/science.273.5274.494
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发表时间：
1996-07-26
期刊：
影响因子：
56.9
通讯作者：
Ried, T
中科院分区：
文献类型：
--
作者：
Schrock, E;duManoir, S;Ried, T
同时和明确的识别所有人类染色体的不同颜色将具有重要的临床和生物学意义。通过光谱核型分析进行全基因组扫描，可以在荧光原位杂交后即时显示每个人类染色体的定义发射光谱，通过计算机分离（分类）光谱，可以分辨光谱重叠的染色体特异性DNA探针，并同时鉴定所有人类染色体。
The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization, By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.