A sphingosine-dependent protein kinase that specifically phosphorylates 14-3-3 (SDK1) is identified as the kinase domain of PKCdelta: a preliminary note.
A sphingosine-dependent protein kinase that specifically phosphorylates 14-3-3 (SDK1) is identified as the kinase domain of PKCdelta: a preliminary note.
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特异性磷酸化 14-3-3 (SDK1) 的鞘氨醇依赖性蛋白激酶被确定为 PKCdelta 的激酶结构域：初步说明。
DOI：
10.1016/s0006-291x(03)01070-2
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发表时间：
2003
影响因子：
3.1
通讯作者：
Hakomori,Senitiroh
中科院分区：
文献类型：
--
作者：
Hamaguchi,Akikazu;Suzuki,Erika;Murayama,Kimie;Fujimura,Tsutomu;Hikita,Toshiyuki;Iwabuchi,Kazuhisa;Handa,Kazuko;Withers,DonaldA;Masters,ShaneC;Fu,Haian;Hakomori,Senitiroh
A specific protein kinase that phosphorylates Ser60, Ser59, or Ser58 of 14-3-3β, η, or ζ, respectively, only in the presence of sphingosine (Sph) or N,N-dimethyl-Sph (DMS), was termed “sphingosine-dependent protein kinase-1” (SDK1) [J. Biol. Chem. 273(34) (1998) 21834]. We have now identified SDK1 as a protein having the same amino acid sequence as in the C-terminal-half kinase domain of PKCδ, with ∼40kDa molecular mass, based on large-scale purification of a protein from rat liver, and partial sequence using three different combinations of LC-MS or LC-MS/MS with respective search engine. PKCδ did not display any SDK1 activity and PKCδ activity was inhibited by Sph and DMS. However, strong SDK1 activity, only in the presence of Sph or DMS, became detectable when PKCδ was incubated with caspase-3, which releases the ∼40kDa kinase domain.
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促凋亡蛋白 Bad 结合 14-3-3zeta 的两亲性凹槽。
DOI：
10.1016/s0167-4838(01)00202-3
发表时间：
2001
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Yang,H;Masters,SC;Wang,H;Fu,H
通讯作者：
Fu,H
影响因子：
4.8
作者：
CORONEOS, E;MARTINEZ, M;KESTER, M
通讯作者：
KESTER, M
影响因子：
7.4
作者：
Zhang, WZ;Chait, BT
通讯作者：
Chait, BT
影响因子：
4.8
作者：
Sano, T;Baker, D;Tigyi, G
通讯作者：
Tigyi, G
影响因子：
2.9
作者：
Edsall, LC;Van Brocklyn, JR;Spiegel, S
通讯作者：
Spiegel, S