EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability.
EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability.
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DOI：
10.1083/jcb.149.4.761
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发表时间：
2000-05-15
期刊：
影响因子：
--
通讯作者：
Bierer BE
中科院分区：
文献类型：
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作者：
Tirnauer JS;Bierer BE
EB1家族代表一组高度保守的蛋白质，存在于酵母中直至人类，定位于纺锤体和细胞质微管，特别是在其远端。芽殖酵母同源EB1，Bim1p，调节微管的稳定性，是重要的定位有丝分裂纺锤体，锚定它的芽通过星形微管附着到皮质蛋白Kar9p。Bim1p在有丝分裂晚期细胞周期检查点中与dynactin功能性相互作用。人细胞中的EB1蛋白与腺瘤性结肠息肉病（APC）肿瘤抑制蛋白发生物理相互作用，将APC靶向微管正末端，并与dynactin复合物的成员发生物理相互作用。在这里，我们回顾了最近的研究表明，在促进微管搜索和捕获和维持染色体稳定性的EB1参与酵母和人类细胞。
The EB1 family represents a highly conserved group of proteins, present in yeast through humans, that localize to spindle and cytoplasmic microtubules, especially at their distal tips. The budding yeast homologue of EB1, Bim1p, regulates microtubule stability and is important for positioning the mitotic spindle, anchoring it to the bud through astral microtubule attachment to the cortical protein Kar9p. Bim1p interacts functionally with dynactin in a late mitotic cell cycle checkpoint. EB1 proteins in human cells interact physically with the adenomatous polyposis coli (APC) tumor suppressor protein, targeting APC to microtubule plus ends, and with members of the dynactin complex. Here, we review recent studies in yeast and human cells that suggest the involvement of EB1 in promoting microtubule search and capture and in maintaining chromosome stability.