IMMUNOHISTOCHEMICAL CHARACTERIZATION OF HEPATIC LYMPHOCYTES IN PRIMARY BILIARY-CIRRHOSIS IN COMPARISON WITH PRIMARY SCLEROSING CHOLANGITIS AND AUTOIMMUNE CHRONIC ACTIVE HEPATITIS
IMMUNOHISTOCHEMICAL CHARACTERIZATION OF HEPATIC LYMPHOCYTES IN PRIMARY BILIARY-CIRRHOSIS IN COMPARISON WITH PRIMARY SCLEROSING CHOLANGITIS AND AUTOIMMUNE CHRONIC ACTIVE HEPATITIS
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DOI：
10.1016/s0025-6196(12)60897-0
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发表时间：
1993-11-01
影响因子：
8.9
通讯作者：
LUDWIG, J
中科院分区：
文献类型：
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作者：
HASHIMOTO, E;LINDOR, KD;LUDWIG, J
我们分析了20例原发性胆汁性肝硬化（PBC），19例原发性硬化性胆管炎，11例自身免疫性慢性活动性肝炎活检标本中肝细胞浸润的免疫表型，通过定量免疫组化方法。具体来说，我们试图确定活化的T细胞，干扰素-γ-生产细胞和自然杀伤细胞。PBC的门脉细胞浸润中含有CD 4细胞，与CD 8细胞相比占优势，CD 4/CD 8比值为2.45：1。碎片状坏死区域的细胞浸润主要含有CD 8细胞。PBC中浸润的CD 8细胞具有细胞毒性（CD 8阳性，CD 11b阴性）细胞的表面表型。大约4%的T细胞表达白细胞介素2受体。干扰素-γ-染色细胞很少被识别（小于2%）。表达自然杀伤细胞标志物CD 16、CD 56或CD 57的细胞很少，约占细胞浸润的5%。PBC和慢性活动性肝炎患者的浸润成分相似。自然杀伤细胞在原发性硬化性胆管炎患者中是正常人的2倍（P
We analyzed the immunophenotypes of hepatic cellular infiltrates by quantitative immunohistochemical methods in biopsy specimens from 20 patients with primary biliary cirrhosis (PBC), 19 with primary sclerosing cholangitis, and 11 with autoimmune chronic active hepatitis. Specifically, we sought to identify activated T cells, interferon-gamma-producing cells, and natural killer cells. The portal cellular infiltrate in PBC contained a preponderance of CD4 cells in comparison with CD8 cells, with a CD4/CD8 ratio of 2.45:1. The cellular infiltrate in areas of piecemeal necrosis contained mostly CD8 cells. Infiltrating CD8 cells in PBC had the surface phenotype of cytotoxic (CD8-positive, CD11b-negative) cells. Approximately 4% of T cells expressed interleukin 2 receptors. Interferon-gamma-staining cells were rarely identified (in less than 2%). Cells that expressed the natural killer cell markers CD16, CD56, or CD57 were infrequent, constituting approximately 5% of the cellular infiltrate. The composition of the infiltrates was similar in patients with PBC and chronic active hepatitis. Natural killer cells were twice as common in patients with primary sclerosing cholangitis (P