Ion specificity in fusion of phosphatidic acid-phosphatidylcholine mixed lipid vesicles.
Ion specificity in fusion of phosphatidic acid-phosphatidylcholine mixed lipid vesicles.
复制标题
磷脂酸-磷脂酰胆碱混合脂质囊泡融合中的离子特异性。
DOI：
10.1016/0005-2736(80)90549-0
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Prestegard,JH
中科院分区：
文献类型：
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作者：
Liao,MJ;Prestegard,JH
磷脂酰胆碱-磷脂酸混合脂质囊泡的融合已被研究的影响下的二价Ca 2+，Mg 2+，Cd 2+，和Ba 2+，其范围从小得多到大得多，比Ca 2+。在Eu 3+的影响下也研究了聚变，Eu 3+具有相似的半径但不同的电荷。这些离子的影响反映在不同程度的融合所确定的囊泡大小的变化，并在不同的融合率监测1H-NMR光谱。Cd 2+的离子半径与Ca 2+相似，对聚变的影响与Ca 2+相同，而其他离子的效率较低。这表明囊泡融合中间体具有理想地适合于Ca 2+或Cd 2+结合的几何形状。
Fusion of phosphatidylcholine-phosphatidic acid mixed lipid vesicles has been studied under the influence of the divalent Ca2+, Mg2+, Cd2+, and Ba2+, which range in size from much smaller to much larger, than Ca2+. Fusion has also been studied under the influence of Eu3+, which has a similar radius but different charge. The effects of these ions are reflected in the different degrees of fusion determined by changes in vesicle size, and in the varying fusion rates monitored by1H-NMR spectroscopy. Cd2+, which has an ionic radius similar to Ca2+, exhibits the same effect on fusion as Ca2+, while other ions show lower efficiencies. This suggests that the vesicle fusion intermediate has a geometry ideally suited to the binding of Ca2+or Cd2+.