Transcription reactivation steps stimulated by oocyte maturation in C. elegans.
Transcription reactivation steps stimulated by oocyte maturation in C. elegans.
复制标题
线虫中卵母细胞成熟刺激的转录再激活步骤。
DOI：
10.1016/j.ydbio.2006.12.039
复制
发表时间：
2007
影响因子：
2.7
通讯作者：
Blackwell,TKeith
中科院分区：
文献类型：
--
作者：
Walker,AmyK;Boag,PeterR;Blackwell,TKeith
Developing oocytes produce materials that will support early embryonic development then cease transcription before fertilization. Later, a distinct transcription program is established in the embryo. Little is understood about how these global gene regulation transitions are effected. We have investigated in C. elegans how oocyte transcription is influenced by maturation, a process that releases meiotic arrest and prepares for fertilization. By monitoring transcription-associated phosphorylation of the RNA polymerase II (Pol II) C-terminal domain (CTD), we find that oocyte transcription shuts down independently of maturation. Surprisingly, maturation signals then induce CTD phosphorylation that is associated specifically with transcription initiation steps and accumulates to high levels when expression of the CTD phosphatase FCP-1 is inhibited. This CTD phosphorylation is also uncovered when a ubiquitylation pathway is blocked, or when maturation is stimulated precociously. CTD phosphorylation is similarly detected during embryonic mitosis, when transcription is also largely silenced. We conclude that oocyte maturation signals induce abortive transcription events in which FCP-1 may recycle phosphorylated Pol II and that analogous processes may occur during mitosis. Our findings suggest that maturation signals may initiate preparations for embryonic transcription, possibly as part of a broader program that begins the transition from maternal to zygotic gene expression.
