The actin-binding proteins adseverin and gelsolin are both highly expressed but differentially localized in kidney and intestine.
The actin-binding proteins adseverin and gelsolin are both highly expressed but differentially localized in kidney and intestine.
复制标题
肌动蛋白结合蛋白阿德维林和凝溶胶蛋白均高表达，但在肾脏和肠道中的定位存在差异。
DOI：
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发表时间：
1998
影响因子：
4
通讯作者：
D. Kwiatkowski
中科院分区：
文献类型：
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作者：
A. Lueck;D. Brown;D. Kwiatkowski
为了了解与肌动蛋白密切相关的肌动蛋白切断蛋白的不同功能，我们使用一组新的高度敏感和特异的抗体试剂，详细研究了这些蛋白在小鼠和人类发育过程中的表达。免疫印迹分析表明，Adserin在小鼠肾脏和肠道各发育阶段均有高表达，在人胎肾和成人肾中也有表达。相反，正如之前报道的那样，明胶蛋白在小鼠和人类组织中的表达要广泛得多。小鼠肾脏切片的免疫组织化学显示附着素和明胶蛋白的定位明显不同。Adserin表达于极周细胞、细肢、粗升肢以及皮质和髓质集合管的主细胞，弥漫分布于细胞质中。明胶蛋白在远曲小管、皮质和髓质集合管的间质细胞、主细胞以及输尿管中均有表达。在远曲小管，明胶蛋白呈弥漫性分布，集合管主细胞定位于基底外侧极。在插入的细胞中，明胶蛋白的定位是不均匀的，要么在顶端，要么弥漫在细胞质中。在胎儿和成人肾脏中，Adserin仅在集合管表达，而明胶蛋白在粗大的升支和集合管中表达。在小鼠和人的肠道中，Adserin在肠上皮细胞中表达，从十二指肠到结肠，从隐窝到绒毛的表达呈递增趋势。这些观察表明，附着素在肾脏和结肠的特定细胞中有高水平的表达，并提示附着素在上皮细胞功能中的一种先前未知的功能。
To understand the distinct functions of the closely related actin-severing proteins adseverin and gelsolin, we examined the expression of these proteins in detail during mouse and human development using a new highly sensitive and specific set of antibody reagents. Immunoblot analysis demonstrated that adseverin was highly expressed in mouse kidney and intestine at all stages of development and in human fetal and adult kidney. In contrast and as reported previously, gelsolin was expressed much more widely in both murine and human tissues. Immunohistochemistry on murine kidney sections revealed a predominantly differential localization of adseverin and gelsolin. Adseverin was expressed in peripolar cells, thin limbs, thick ascending limbs, and principal cells of cortical and medullary collecting ducts where it was diffusely localized in the cytoplasm. Gelsolin was expressed in the distal convoluted tubule, intercalated cells and principal cells of cortical and medullary collecting ducts, and in ureter. In the distal convoluted tubule, gelsolin showed a diffuse distribution and in principal cells of collecting ducts a localization at the basolateral pole. In intercalated cells, gelsolin localization was heterogeneous, either at the apical pole or diffusely in the cytoplasm. In human fetal and adult kidney, adseverin was expressed only in collecting ducts whereas gelsolin was expressed in thick ascending limbs and collecting ducts. In mouse and human intestine adseverin was expressed in enterocytes with a gradient of increasing expression from the duodenum to the colon, and from the crypt to the villus. The observations indicate high level expression of adseverin in specific cells of the kidney and colon, and suggest a previously unrecognized function of adseverin in epithelial cell function.