Induction of a heat shock response (HSP 72) in rat embryos exposed to selected chemical teratogens.
Induction of a heat shock response (HSP 72) in rat embryos exposed to selected chemical teratogens.
复制标题
暴露于选定的化学致畸剂的大鼠胚胎中诱导热休克反应（HSP 72）。
DOI：
10.1002/tera.1420490209
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Cornel,L
中科院分区：
文献类型：
--
作者：
Mirkes,PE;Doggett,B;Cornel,L
使用针对72 kD热休克蛋白（HSP 72）的单克隆抗体、Western印迹分析和2-D凝胶电泳/放射自显影来确定选定的化学致畸剂是否诱导体外暴露的着床后大鼠胚胎中HSP 72的合成和积累。研究的化学致畸剂包括N-乙酰氧基-2-乙酰氨基芴（N-Ac-AAF）、氯化镉（CAD）、环磷酰胺（CP）、亚砷酸钠（AS）和水杨酸钠（SAL）。选择暴露于试验化学品，产生明显的胚胎毒性，特征为异常发育和生长迟缓。在所研究的五种化学致畸剂中，AS和SAL诱导了10天大鼠胚胎HSP 72的合成和积累。然而，AS和SAL处理的胚胎之间HSP 72积累的动力学不同。在AS暴露后24小时和SAL暴露后10小时观察到最大水平的HSP 72。N-Ac-AAF、CD和CP诱导了明显的胚胎毒性;然而，在任何测定的时间点，这些化学致畸剂均未诱导HSP 72。虽然只有一个小样本的化学致畸剂进行了研究，我们的研究结果表明，热休克反应，其特点是HSP 72的合成和积累，是不是一个通用的生物标志物的化学致畸剂。© 1994 Wiley利斯公司
A monoclonal antibody to the 72 kD heat shock protein (HSP 72), Western blot analysis and 2‐D gel electrophoresis/autoradiography were used to determine whether selected chemical teratogens induced the synthesis and accumulation of HSP 72 in postimplantation rat embryos exposed in vitro. The chemical teratogens studied include N‐Acetoxy‐2‐acetylaminofluorene (N‐Ac‐AAF), cadmium chloride (CAD), cyclophosphamide (CP), sodium arsenite (AS), and sodium salicylate (SAL). Exposures to test chemicals were selected that produced obvious embryotoxicity characterized by abnormal development and growth retardation. Of the five chemical teratogens studied, AS and SAL induced the synthesis and accumulation of HSP 72 in day 10 rat embryos. The kinetics of HSP 72 accumulation, however, differed between AS‐ and SAL‐treated embryos. Maximal levels of HSP 72 were observed 24 hours after AS exposure and 10 hours after SAL exposure. N‐Ac‐AAF, CD, and CP induced obvious embryotoxicity; however, none of these chemical teratogens induced HSP 72 at any of the timepoints assayed. Although only a small sample of chemical teratogens was studied, our results suggest that the heat shock response, characterized by the synthesis and accumulation of HSP 72, is not a general biomarker for chemical teratogens. © 1994 Wiley‐Liss, Inc.