Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer
Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer
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DOI：
10.1021/ac015655c
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发表时间：
2002-03-01
影响因子：
7.4
通讯作者：
Shevchenko, A
中科院分区：
文献类型：
--
作者：
Ekroos, K;Chernushevich, IV;Shevchenko, A
采用具有离子捕获功能的混合四极杆飞行时间质谱仪对内源性磷脂的总提取物进行定量分析。多个碎片离子的前体离子光谱的同时采集允许在单个实验中检测主要类别的磷脂。使用同位素标记的内源性脂质的混合物作为全面的内标，监测其浓度的相对变化。前体离子扫描光谱同时获得的主要脂肪酸的酰基阴离子在负离子模式和确定的脂肪酸部分和它们的相对位置在甘油主链在个别脂质物种。总之，多个前体离子扫描的组合允许定量监测磷脂组成中的主要扰动，并阐明单个脂质物质的分子异质性。
A hybrid quadrupole time-of-flight mass spectrometer featured with ion trapping capabilities was employed for quantitative profiling of total extracts of endogenous phospholipids. Simultaneous acquisition of precursor ion spectra of multiple fragment ions allowed detection of major classes of phospholipids in a single experiment. Relative changes in their concentration were monitored using a mixture of isotopically labeled endogenous lipids as a comprehensive internal standard. Precursor ion scanning spectra were acquired simultaneously for acyl anions of major fatty acids in negative ion mode and identified the fatty acid moieties and their relative position at the glycerol backbone in individual lipid species. Taken together, a combination of multiple precursor ion scans allowed quantitative monitoring of major perturbation in phospholipid composition and elucidating of molecular heterogeneity of individual lipid species.