Association of transforming growth factor β1 genotype with spinal osteophytosis in Japanese women
Association of transforming growth factor β1 genotype with spinal osteophytosis in Japanese women
复制标题
DOI：
10.1002/1529-0131(200002)43:2
复制
发表时间：
2000-02-01
影响因子：
--
通讯作者：
Harada, A
中科院分区：
文献类型：
--
作者：
Yamada, Y;Okuizumi, H;Harada, A
目标。为探讨转化生长因子β1基因第29位T-->；C多态与腰椎放射型骨赘病遗传易感性的关系，对540名绝经后日本妇女进行了脊柱X线片和腰椎及全身骨密度测定。对67例骨质疏松症和脊柱骨质疏松症患者的腰椎间盘进行磁共振成像(MRT)检查，并用等位基因特异性聚合酶链式反应方法检测转化生长因子β1(TGF-β1)基因分型。测定29例正常对照组和36例脊柱骨赘患者的血清转化生长因子β1浓度。在所有研究对象中，CC基因携带者的放射学脊柱骨赘患病率高于TC或TT基因携带者。Logistic回归分析表明，在调整了年龄、身高、体重、绝经时间、吸烟状况、体脂、瘦质量以及腰椎或全身骨密度后，脊柱骨赘患者的C等位基因频率显著高于未患此病的患者。在对照组、骨质疏松症组和脊柱骨赘病组之间的比较也显示，骨赘病组中C等位基因比对照组更常见，即使在调整了BMD后也是如此，相反，如前所述，骨质疏松症患者C等位基因的频率低于对照组。CC基因携带者的腰椎间盘面积和腰椎面积/椎体面积比值也是最低的，无论是对照组还是脊柱骨赘患者，血清TGFP1浓度均随C等位基因数目的增加而升高。转化生长因子β1基因的T-29-->；C多态与脊柱骨赘和骨质疏松症的遗传易感性呈反比关系。虽然骨赘的放射学评估可能不能反映实际的疾病严重程度，但预防骨质疏松的C等位基因可能是脊柱骨赘病遗传易感性的危险因素。
Objective. To examine the possible relationship between a T-->C polymorphism at nucleotide position 29 of the transforming growth factor beta 1 (TGF beta 1) gene and genetic susceptibility to radiographic spinal osteophytosis,Methods, A total of 540 postmenopausal Japanese women were subjected to radiography of the spine and determination of bone mineral density (BMD) for the lumbar spine and total body. Changes in lumbar intervertebral discs were examined in 67 individuals with either osteoporosis or spinal osteophytosis by magnetic resonance imaging (MRT), TGF beta 1 genotype was determined with an allele-specific polymerase chain reaction assay. The serum concentration of TGF beta 1 was measured in 29 control subjects and in 36 patients with spinal osteophytosis.Results. Among all study subjects, the prevalence of radiographic spinal osteophytosis in individuals with the CC genotype was greater than that in those with the TC or TT genotype, Logistic regression analysis, adjusted for age, height, body weight, time since menopause, smoking status, body fat, lean mass, and either lumbar spine or total body BMD, demonstrated that the frequency of the C allele in subjects with spinal osteophytosis was significantly greater than that in those without this condition. Comparison among control, osteoporosis, and spinal osteophytosis groups also revealed that the C allele was more prevalent in subjects with osteophytosis than in controls, even after adjustment for BMD, In contrast, as previously shown, the frequency of the C allele was lower in osteoporosis patients than in controls. The intervertebral disc area and the ratio of disc area to vertebral body area, as determined by MRI, were also lowest in subjects with the CC genotype, The serum concentration of TGFP1 increased with the number of C alleles in both controls and patients with spinal osteophytosis.Conclusion. The T-29-->C polymorphism of the TGF beta 1 gene exhibited inverse patterns of association with genetic susceptibility to spinal osteophytosis and with osteoporosis. Although radiographic evaluation of osteophytes might not reflect the actual disease severity, the C allele, which protects against osteoporosis, may be a risk factor for genetic susceptibility to spinal osteophytosis.