Breeding, husbandry, veterinary care, and hematology of marsh rice rats (Oryzomys palustris), a small animal model for periodontitis.
Breeding, husbandry, veterinary care, and hematology of marsh rice rats (Oryzomys palustris), a small animal model for periodontitis.
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发表时间：
2015
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通讯作者：
J. Aguirre;K. Edmonds;B. Zamora;Jennifer E. Pingel;L. Thomas;Denisse Cancel;Laura Schneider;M. Reinhard;A. Battles;M. Akhter;D. Kimmel;T. Wronski
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作者：
J. Aguirre;K. Edmonds;B. Zamora;Jennifer E. Pingel;L. Thomas;Denisse Cancel;Laura Schneider;M. Reinhard;A. Battles;M. Akhter;D. Kimmel;T. Wronski
水稻大鼠（Oryzomys palustris）是一种公认的动物模型，用于研究牙周疾病和光周期调节的生殖。在这里，我们分享了关于该动物物种的育种，饲养，兽医护理和血液学发现的信息，以促进其在其他研究机构的研究中的使用。稻鼠最初被隔离，并通过使用微生物学，寄生虫学和血清学方法与成年雌性小家鼠和褐家鼠哨兵动物监测排除的病原体。繁殖者在一夫一妻制的连续繁殖系统中配对。将大鼠饲养在静态过滤盖笼中，在68至79 °F（20.0至26.1 °C）和30%至70%湿度下，在14：10-h光照：黑暗循环下维持商业饲料。尽管稻鼠对同种和人类有攻击行为，但它们显然对标准实验室条件适应得相对较好。我们对97窝的分析表明，母鼠平均每只母鼠产下5.2只幼崽，每只母鼠断奶4.2只幼崽。通常用于标准实验室啮齿动物（小鼠和大鼠）的几种程序和生物试剂可用于水稻大鼠。此外，我们目前的血液学和血清化学值，可作为初步的参考值，为未来的研究涉及水稻大鼠。
Rice rats (Oryzomys palustris) are a recognized animal model for studying periodontal disease and the photoperiodic regulation of reproduction. Here we share information regarding the breeding, husbandry, veterinary care, and hematologic findings about this animal species to facilitate its use in studies at other research institutions. Rice rats initially were quarantined and monitored for excluded pathogens by using microbiologic, parasitologic, and serologic methods with adult female Mus musculus and Rattus norvegicus sentinel animals. Breeders were paired in a monogamous, continuous-breeding system. Rats were housed in static filter-top cages, maintained on commercial chow under 14:10-h light:dark cycles at 68 to 79 °F (20.0 to 26.1 °C) and 30% to 70% humidity. Rice rats apparently adapt relatively well to standard laboratory conditions, despite their aggressive behavior toward conspecifics and humans. Our analysis of 97 litters revealed that dams gave birth to an average of 5.2 pups per dam and weaned 4.2 pups per dam. Several procedures and biologic reagents normally used in standard laboratory rodents (mice and rats) can be used with rice rats. In addition, we present hematologic and serum chemistry values that can be used as preliminary reference values for future studies involving rice rats.