Controlled delivery of bone morphogenic protein-2-related peptide from mineralised extracellular matrix-based scaffold induces bone regeneration
Controlled delivery of bone morphogenic protein-2-related peptide from mineralised extracellular matrix-based scaffold induces bone regeneration
复制标题
从矿化细胞外基质支架中受控递送骨形态发生蛋白2相关肽可诱导骨再生
DOI：
10.1016/j.msec.2021.112182
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发表时间：
2021-05-19
影响因子：
7.9
通讯作者：
Sun, Tingfang
中科院分区：
文献类型：
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作者：
Meng, Chunqing;Su, Weijie;Sun, Tingfang
理想的骨组织工程支架材料是由细胞外基质（ECM）组成，具有良好的模拟骨环境的骨传导能力。我们开发了一种矿化组织衍生的ECM修饰的真骨陶瓷（TBC）支架，用于递送天冬氨酸修饰的骨形态发生蛋白-2（BMP-2）肽（P28），并评估其成骨能力。将猪小肠粘膜下层（SIS）脱细胞ECM包被在TBC表面，然后进行矿化修饰（mSIS/TBC）。随后在不存在交联剂的情况下将P28固定到支架上。碱性磷酸酶活性和其他成骨分化标志物结果显示，P28/mSIS/TBC支架的成骨作用显著大于TBC和mSIS/TBC组。此外，为了检测该系统在体内的骨传导能力，我们建立了大鼠颅骨缺损模型，并评估了新骨面积和新血管密度。组织学观察显示P28/mSIS/TBC具有良好的骨再生效果。本研究提出使用负载P28的mSIS/TBC作为骨组织工程应用的有希望的成骨支架。
Ideal bone tissue engineering scaffolds composed of extracellular matrix (ECM) require excellent osteoconductive ability to imitate the bone environment. We developed a mineralised tissue-derived ECM-modified true bone ceramic (TBC) scaffold for the delivery of aspartic acid-modified bone morphogenic protein-2 (BMP-2) peptide (P28) and assessed its osteogenic capacity. Decellularized ECM from porcine small intestinal submucosa (SIS) was coated onto the surface of TBC, followed by mineralisation modification (mSIS/TBC). P28 was subsequently immobilised onto the scaffolds in the absence of a crosslinker. The alkaline phosphatase activity and other osteogenic differentiation marker results showed that osteogenesis of the P28/mSIS/TBC scaffolds was significantly greater than that of the TBC and mSIS/TBC groups. In addition, to examine the osteoconductive capability of this system in vivo, we established a rat calvarial bone defect model and evaluated the new bone area and new blood vessel density. Histological observation showed that P28/mSIS/TBC exhibited favourable bone regeneration efficacy. This study proposes the use of mSIS/TBC loaded with P28 as a promising osteogenic scaffold for bone tissue engineering applications.