Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World
Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World
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DOI：
10.1016/s0001-706x(01)00215-7
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发表时间：
2002-03-01
期刊：
影响因子：
2.7
通讯作者：
Hashiguchi, Y
中科院分区：
文献类型：
--
作者：
Mimori, T;Matsumoto, T;Hashiguchi, Y
在这项研究中，我们测试了聚合酶链反应（PCR）的方法来诊断皮肤利什曼病（CL），采取渗出物材料与棉花拭子病变，使用我们以前测试（PCR）面板组成的利什曼原虫（Viannia）巴拿马，L。（五）braziliensis、巴西湖蝇L.（五）guyanensis，L.（Leishmania）mexicana和L.（L.）亚马逊河。本研究的目的是改进方便患者的采样方法，并检验棉拭子采样的有效性。16例患者被临床诊断为CL，包括一例弥漫性皮肤利什曼病（DCL）在厄瓜多尔和致病利什曼原虫寄生虫进行了鉴定。12份利什曼原虫DNA阳性的拉马纳CL患者标本经鉴定均为利什曼原虫。（五）Panamensis，Huigra的CL和San伊格纳西奥的DCL分别有2株和1株为L.（L.）墨西哥。在现场条件下，取活检材料不仅疼痛，而且有时会导致医源性细菌感染。考虑到试验的敏感性和方便的采样程序，可以建议使用棉签收集渗出液可能是用于CL PCR诊断的活检样本的更好的替代方案。(C)2002年由Elsevier Science B.V.出版
In this study, we tested the polymerase chain reaction (PCR)-method to diagnose cutaneous leishmaniasis (CL) by taking exudate materials from lesions with cotton swabs, using our previously tested (PCR) panel comprised of Leishmania (Viannia) panamensis, L. (V.) braziliensis, L. (V.) guyanensis, L. (Leishmania) mexicana and L. (L.) amazonensis. The objectives of the present study were to improve the sampling method convenient for the patients and to test the usefulness of samples taken with cotton swabs. Sixteen patients were clinically diagnosed to have CL including one case of diffuse cutaneous leishmaniasis (DCL) in Ecuador and the causative Leishmania parasites were identified by PCR. All the 12 samples from CL patients of La Mana, positive for Leishmania DNA, were identified as L. (V.) panamensis, while two from CL of Huigra and one from DCL of San Ignacio were L. (L.) mexicana. In the field condition, taking biopsy material is not only painful but sometimes causes iatrogenic bacterial infections. Considering the sensitivity of the test, and convenient sampling procedure, it may be suggested that collection of exudates using cotton swabs may be a better alternative to biopsy sample for PCR-diagnosis of CL. (C) 2002 Published by Elsevier Science B.V.