Background subtraction in experimental data arrays illustrated by the example of Raman spectra and fluorescent gel electrophoresis patterns
Background subtraction in experimental data arrays illustrated by the example of Raman spectra and fluorescent gel electrophoresis patterns
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DOI：
10.1023/b:inet.0000008930.87741.b9
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发表时间：
2003-11-01
影响因子：
0.6
通讯作者：
Razzhivin, AP
中科院分区：
文献类型：
--
作者：
Mikhailyuk, IK;Razzhivin, AP
从一维实验数据阵列（光谱、色谱图）和二维阵列（光谱图）中减去背景信号的方法是基于有用信号和背景信号的曲线的几何特性（曲率半径）的差异。为了证明这种方法的有效性，它被应用到胰凝乳蛋白酶的拉曼光谱和荧光凝胶电泳的B肝炎DNA片段用溴化乙锭染色。
A method for background signal subtraction from one-dimensional experimental data arrays (optical spectra, chromatograms) and two-dimensional arrays (electrophoregrams) is based on the difference in the geometric properties (radii of curvature) of the curves of the useful and background signals. To demonstrate the efficiency of this method, it is applied to the Raman spectrum of chymotrypsin and a fluorescent gel electrophoregram of hepatitis B DNA fragments stained with ethydium bromide.