Methyl parathion hydrolase based nanocomposite biosensors for highly sensitive and selective determination of methyl parathion
Methyl parathion hydrolase based nanocomposite biosensors for highly sensitive and selective determination of methyl parathion
复制标题
基于甲基对硫磷水解酶的纳米复合生物传感器，用于高灵敏度和选择性测定甲基对硫磷
DOI：
10.1016/j.bios.2011.04.025
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发表时间：
2011-07-15
影响因子：
12.6
通讯作者：
Zhang, Aidong
中科院分区：
文献类型：
--
作者：
Chen, Shizhen;Huang, Jing;Zhang, Aidong
本文报道了一种用于甲基对硫磷灵敏、特异检测的纳米复合生物传感器的研制。通过在二氧化硅颗粒上形成金纳米颗粒，与多壁碳纳米管混合，然后共价固定化甲基对硫磷水解酶，制备了纳米复合传感薄膜。各个材料的复合被微调，以提供具有高比表面积和高电导率的传感薄膜。在甲基对硫磷生物传感中，纳米复合材料对生物传感器的性能有显著的协同效应。方波伏安响应峰清晰，与甲基对硫磷的浓度在0.001~5.0mgmL(-1)范围内呈线性关系，检出限为0.3ngmL(-1)。并对该生物传感器在大蒜样品分析中的应用进行了评价。该方法可作为一种简单、快速构建生物传感器的平台，具有良好的性能，可用于酶特异性电活性物种的检测。(C)2011爱思唯尔B.V.保留所有权利。
This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was prepared via the formation of gold nanoparticles on silica particles, mixing with multiwall carbon nanotubes and subsequent covalent immobilization of methyl parathion hydrolase. The composite of the individual materials was finely tuned to offer the sensing film with high specific surface area and high conductivity. A significant synergistic effect of nanocomposites on the biosensor performance was observed in biosensing methyl parathion. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentrations of methyl parathion in the range from 0.001 mu g mL(-1) to 5.0 mu g mL(-1) with a detection limit of 0.3 ng mL(-1). The application of this biosensor in the analysis of spiked garlic samples was also evaluated. The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species. (C) 2011 Elsevier B.V. All rights reserved.