Molded rigid polymer monoliths as separation media for capillary electrochromatography
Molded rigid polymer monoliths as separation media for capillary electrochromatography
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DOI：
10.1021/ac970377w
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发表时间：
1997-09-01
影响因子：
7.4
通讯作者：
Frechet, JMJ
中科院分区：
文献类型：
--
作者：
Peters, EC;Petro, M;Frechet, JMJ
用于反相电色谱的刚性整体毛细管柱在未处理的熔融石英毛细管范围内通过二甲基丙烯酸乙二酯、甲基丙烯酸丁酯和 2-丙烯酰氨基-2-甲基-1-丙磺酸在致孔剂存在下的简单共聚一步制备而成。特别设计的三元致孔溶剂的组成可以在较宽的范围内精细控制整体材料的多孔性质。虽然现在通过这些毛细管柱的电渗随着整料孔径和带电官能团含量的增加而增加，但对于具有更大表面积和疏水性的整料，观察到更好的色谱性质。利用该技术，可以轻松获得效率高于 120 000 板/米的整体式毛细管柱。
Rigid, monolithic capillary columns for reversed-phase electrochromatography have been prepared within the confines of untreated fused-silica capillaries in a single step by a simple copolymerization of ethylene dimethacrylate, butyl methacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogenic soh ent. The composition of the specifically designed ternary porogenic solvent allows fine control of the porous properties of the monolithic material over a broad range. While the electroosmotic now through these capillary columns increases with both increasing pore size of the monolith and content of charged functionalities, better chromatographic properties have been observed for monoliths with larger surface area and hydrophobicity. Using this technique, monolithic capillary columns with an efficiency higher than 120 000 plates/m have been easily obtained.