DOPAMINERGIC CELLS OF THE RIVER LAMPREY RETINA REVEALED BY A HISTOFLUORESCENCE STUDY
DOPAMINERGIC CELLS OF THE RIVER LAMPREY RETINA REVEALED BY A HISTOFLUORESCENCE STUDY
复制标题
组织荧光研究揭示七鳃鳗视网膜的多巴胺能细胞
DOI：
10.1267/ahc.15.757
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
T. Teranishi
中科院分区：
文献类型：
--
作者：
K. Negishi;S. Kato;T. Teranishi
采用平板显微镜和冷冻显微镜对江七鳃鳗（Lampetra japonica）视网膜进行荧光显微镜研究。玻璃体内注射去甲肾上腺素（2.5μg）和5,6 -二羟色胺（2.5μg）的混合物2h后去核。计算视网膜平面支架任意四个同心圆区域的多巴胺能（DA）细胞密度（数量/mm2）；中心、中间、外围和边缘区域。发现密度较高（平均值±标准差）= 131±29个细胞/mm2；n=12)外周区比其他区多，边缘区极低（15±7个细胞/mm2），紧靠终末口的周缘无DA细胞存在。卵圆形或梭形DA细胞向各个方向延伸2 ~ 3个主要突起，在无毛细胞和内丛状层不规则分叉，形成致密的纤维网络。由于水平细胞层未见荧光纤维，因此DA细胞可能是七鳃鳗视网膜中无突细胞的一个亚群。讨论了七鳃鳗和硬骨鱼视网膜DA细胞在形态和空间分布上的差异。
The retinas of the river lamprey (Lampetra japonica) were processed for fluorescence microscopic study with flat-mounts and cryosections. The eyes were enucleated 2hr after an intravitreous injection of a mixture of noradrenaline (2.5μg) and 5, 6-dihydroxytryptamine (2.5μg). The density (number/mm2) of dopaminergic (DA) cells was calculated for four arbitrary concentric regions of retinal flat-mounts; the central, intermediate, peripheral and marginal regions. The density was found to be higher (mean±S.D.= 131±29 cells/mm2; n=12) in the peripheral region than in the rest and extremely low (15±7cells/mm2) in the marginal region, and no DA cells existed in the circumferential edge immediately neighboring the ora terminalis. Oval or fusiform DA cells extended in various directions 2 to 3 main processes, which irregularly ramified and formed dense fiber networks in the amacrine cell and inner plexiform layers. Since no fluorescent fiber was seen in the horizontal cell layer, DA cells appeared to be a subset of amacrine cells in the lamprey retina. Differences in morphology and spatial distribution were discussed between DA cells of the lamprey and teleost fish retinas.