Caudal, a key developmental regulator, is a DPE-specific transcriptional factor
Caudal, a key developmental regulator, is a DPE-specific transcriptional factor
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DOI：
10.1101/gad.1698108
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发表时间：
2008-10-15
影响因子：
10.5
通讯作者：
Kadonaga, James T.
中科院分区：
文献类型：
--
作者：
Juven-Gershon, Tamar;Hsu, Jer-Yuan;Kadonaga, James T.
基因转录的调节对于生物体的正常发育和生长至关重要。蛋白质编码基因的转录起始于RNA聚合酶II核心启动子，这是一个多样化的模块，可以由许多不同的元件控制，如TATA盒和下游核心启动子元件（DPE）。为了了解核心启动子多样性的基础，我们探索了DPE的潜在生物学功能。我们发现，几乎所有的果蝇同源异型（Hox）基因启动子，缺乏TATA盒元件，含有功能上重要的DPE基序，是保守的果蝇黑腹果蝇，果蝇virilis。然后，我们发现，Caudal，一个序列特异性转录因子和Hox基因网络的关键调节因子，激活转录，相对于TATA盒，对DPE有明显的偏好。当BREu核心启动子基序与TATA盒相关时，尾部激活对DPE的特异性特别引人注目。这些发现表明，Caudal是一种DPE特异性激活剂，并且其核心启动子多样性可用于建立复杂的调控网络。
The regulation of gene transcription is critical for the proper development and growth of an organism. The transcription of protein-coding genes initiates at the RNA polymerase II core promoter, which is a diverse module that can be controlled by many different elements such as the TATA box and downstream core promoter element (DPE). To understand the basis for core promoter diversity, we explored potential biological functions of the DPE. We found that nearly all of the Drosophila homeotic (Hox) gene promoters, which lack TATA-box elements, contain functionally important DPE motifs that are conserved from Drosophila melanogaster to Drosophila virilis. We then discovered that Caudal, a sequence-specific transcription factor and key regulator of the Hox gene network, activates transcription with a distinct preference for the DPE relative to the TATA box. The specificity of Caudal activation for the DPE is particularly striking when a BREu core promoter motif is associated with the TATA box. These findings show that Caudal is a DPE-specific activator and exemplify how core promoter diversity can be used to establish complex regulatory networks.