The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin.
The expression of p120ctn protein in breast cancer is independent of alpha- and beta-catenin and E-cadherin.
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发表时间：
1998
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通讯作者：
Deborah A. Dillon;T. D'Aquila;Albert B. Reynolds;Eric R. Fearon;D. Rimm
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作者：
Deborah A. Dillon;T. D'Aquila;Albert B. Reynolds;Eric R. Fearon;D. Rimm
一些研究已经报道了E-钙粘蛋白在乳腺癌中的表达缺失或改变，以及最近连环蛋白表达水平的变化。应用免疫荧光技术检测91例浸润性导管癌组织中E-钙粘蛋白、α-连环素、β-连环素和p120ctn(原p120CAS)的表达。正如预期的那样，所有四种蛋白质都共定位于细胞的连接区。虽然在结肠息肉中已经描述了β-连环素的核定位，但在这些乳腺癌病例中没有发现这种例子。我们发现，尽管连环蛋白和E-钙粘蛋白的改变是常见的，但完全丢失，如E-钙粘蛋白在小叶癌中的例子(E-钙粘蛋白经常突变)，很少见。相反，连接素相关蛋白p120ctn的表达模式与其他连接素(或E-钙粘蛋白)显著无关，包括在大约10%的病例中完全丧失表达。没有观察到与传统预后指标有统计学意义的相关性，这些蛋白中的任何一个。我们得出的结论是：1)E-钙粘蛋白和α-和β-连环素的表达通常保留在细胞膜上，但经常减少或改变；2)p120ctn的表达在大约10%的病例中完全丧失；3)E-钙粘附素和连环素的表达存在显著的相关性，但这些分子与p120ctn之间没有相关性，提示缺乏协调调控。
Several studies have reported loss or alteration of expression of E-cadherin in breast cancer and more recently changes in levels of expression of the catenins. We used immunofluorescence to examine E-cadherin, alpha-catenin, beta-catenin, and p120ctn (formerly p120CAS) expression in 91 cases of invasive ductal carcinoma. As expected, all four proteins co-localize to the junctional regions of the cells. Although nuclear localization has been described for beta-catenin in colonic polyps, no examples were found in these breast cancer cases. We found that, although alteration is common in the catenins and E-cadherin, complete loss, as exemplified by E-cadherin in lobular carcinoma (where E-cadherin is frequently mutated), is rarely seen. In contrast, the catenin-related protein p120ctn shows an expression pattern that is significantly unrelated to the other catenins (or E-cadherin), including complete loss of expression in approximately 10% of the cases. No statistically significant correlations with traditional prognostic indicators were observed with any of these proteins. We conclude 1) that expression of E-cadherin and alpha- and beta-catenin are generally retained at the membrane although frequently reduced or altered, 2) that complete loss of p120ctn expression is seen in approximately 10% of the cases, and 3) that there is a significant correlation in the expression of E-cadherin and the catenins but no correlation between these molecules and p120ctn, suggesting an absence of coordinate regulation.