Lack of evidence for neutrophil participation during infarct formation following focal cerebral ischemia in the rat
Lack of evidence for neutrophil participation during infarct formation following focal cerebral ischemia in the rat
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DOI：
10.1006/exnr.1996.0093
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发表时间：
1996-06-01
影响因子：
5.3
通讯作者：
Bartus, RT
中科院分区：
文献类型：
--
作者：
Hayward, NJ;Elliott, PJ;Bartus, RT
The involvement of neutrophils in the pathogenesis of cerebral ischemic injury in two rat models of focal ischemia was investigated. In Experiment I, a model of focal ischemia with partial reperfusion was used. Although significant and discrete ischemic damage within the neocortex was nearly maximal at 12 h postocclusion, no elevation in neutrophils was seen at this time point. Even after 21 h postocclusion, only a subtle increase in neutrophils within the ischemic tissue was observed. To further investigate the possible role of neutrophils in cerebral ischemia, the effect of cyclophosphamide-induced neutropenia was investigated (Experiment II). While a marked reduction (>98%) in systemic neutrophils was achieved in advance of and during the ischemic challenge, no reduction in the volume of ischemic damage was observed. In Experiment III, variations in the rat model of focal ischemia were made to produce a larger area of ischemic damage, as well as to permit; complete reperfusion of blood to the affected cortex. While more neutrophils were seen in this variation of the model, very few were observed (
