High sensitive detection method for protein by combining the magnetic separation with cation exchange based signal amplification
High sensitive detection method for protein by combining the magnetic separation with cation exchange based signal amplification
复制标题
磁分离与阳离子交换信号放大相结合的高灵敏度蛋白质检测方法
DOI：
10.1016/j.talanta.2017.03.018
复制
发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Xie Hai-yan
中科院分区：
文献类型：
--
作者：
Xu Jin;Zhang Qian-mei;Zhao Dong-xu;Liu Ya-ru;Chen Ping;Lu Gui-hong;Xie Hai-yan
PSA 是低丰度蛋白质的成员，是前列腺癌发展和治疗效果的关键指标。在本研究中，通过集成免疫磁性分离和基于阳离子交换的信号放大，开发了一种简便且高灵敏度的血清 PSA 检测方法。在纳米颗粒制备和免疫探针构建的基础上，通过免疫磁性探针捕获、分离血清中的PSA，然后与基于量子点（QDs）的免疫荧光探针相互作用； QD 内的 Zn2+ 在几秒钟内被 Ag+ 取代，之后荧光信号被 Zn2+ 响应染料 Fluozin-3 放大。在优化条件下，检测限低（1.56 pg/mL），线性范围宽（1.56–25 ng/mL），重复性好（系数内变异=3.18%），优于商业化ELISA试剂盒。这些结果证明了我们的高灵敏度 PSA 检测方法在临床中的潜力。
PSA is a member of low abundance proteins and serves as a critical indicator of the development and therapy efficacy for prostate cancer. In this study, a facile and high sensitive method was developed for serum PSA detection by integrating the immunomagnetic separation and cation exchange based signal amplification. On the basis of nanoparticle preparation and immunoprobe construction, PSA in serum was captured, separated by the immunomagnetic probe and then interacted with the quantum dots (QDs) based immunofluorescence probe; Zn2+inside QDs was replaced by Ag+within seconds, after which fluorescence signal was amplified by Fluozin-3, the Zn2+responsive dye. Under optimized conditions, low detection limit (1.56 pg/mL), wide linear range (1.56–25 ng/mL) and good repeatability (intra-coefficient variation=3.18%) were achieved, which is superior to commercialized ELISA kit. These results demonstrated the potential of our high sensitive method for PSA detection in clinical.