Macropinocytosis: searching for an endocytic identity and role in the uptake of cell penetrating peptides.
Macropinocytosis: searching for an endocytic identity and role in the uptake of cell penetrating peptides.
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DOI：
10.1111/j.1582-4934.2007.00062.x
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发表时间：
2007-07
影响因子：
5.3
通讯作者：
Jones AT
中科院分区：
文献类型：
--
作者：
Jones AT
大型细胞增多症定义了一系列事件，该事件是由广泛的质膜重组或荷叶边形成外部大型细胞结构的一系列事件，然后将其封闭和内在化。在某些生物体和细胞类型中，该过程是构成的，但在另一些生物体中，该过程仅在生长因子刺激后才发音。内在的大型大体与吞噬体共享许多特征，并且两者都通过大小，形态异质性和缺乏外套结构而与其他形式的皮细胞囊泡区别开来。关于大胞刺病的其他区别特征，例如特定的标记蛋白和药物，这些信息缺乏信息，这些特征会干扰其在其他内吞过程中的机制。这阻碍了表征该途径动力学的努力，并确定表达以允许其进行的调节蛋白。内在化后，大肺泡会获得其他内吞途径共有的调节蛋白，这表明它们作为独特结构的身份是短暂的。然而，关于大型货物的总体命运或其极限膜以及融合，管道，回收和调节的胞吐作用等过程的共识均涉及塑造大型骨质体和导向货物的交通。大型细胞增多症也与细胞穿透性肽的内在化有关，这些肽的内在化旨在利用其易位能力将基因和蛋白质等治疗实体传递到细胞中。这篇综述着重于有关大型细胞增多症的调节的最新发现，这是大型细胞体的细胞内命运，并讨论了该途径作为细胞穿透性肽入境机理的作用的证据。
Macropinocytosis defines a series of events initiated by extensive plasma membrane reorganization or ruffling to form an external macropinocytic structure that is then enclosed and internalized. The process is constitutive in some organisms and cell types but in others it is only pronounced after growth factor stimulation. Internalized macropinosomes share many features with phagosomes and both are distinguished from other forms of pinocytic vesicles by their large size, morphological heterogeneity and lack of coat structures. A paucity of information is available on other distinguishing features for macropinocytosis such as specific marker proteins and drugs that interfere with its mechanism over other endocytic processes. This has hampered efforts to characterize the dynamics of this pathway and to identify regulatory proteins that are expressed in order to allow it to proceed. Upon internalization, macropinosomes acquire regulatory proteins common to other endocytic pathways, suggesting that their identities as unique structures are short-lived. There is however less consensus regarding the overall fate of the macropinosome cargo or its limiting membrane and processes such as fusion, tubulation, recycling and regulated exocytosis have all been implicated in shaping the macropinosome and directing cargo traffic. Macropinocytosis has also been implicated in the internalization of cell penetrating peptides that are of significant interest to researchers aiming to utilize their translocation abilities to deliver therapeutic entities such as genes and proteins into cells. This review focuses on recent findings on the regulation of macropinocytosis, the intracellular fate of the macropinosome and discusses evidence for the role of this pathway as a mechanism of entry for cell penetrating peptides.
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影响因子：
9.2
作者：
Bretscher, MS;Aguado-Velasco, C
通讯作者：
Aguado-Velasco, C
DOI：
10.1073/pnas.1735556100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Ackerman, AL;Kyritsis, C;Cresswell, P
通讯作者：
Cresswell, P
影响因子：
3.3
作者：
Amyere, M;Payrastre, B;Courtoy, PJ
通讯作者：
Courtoy, PJ
影响因子：
4.5
作者：
Clarke, M;Köhler, J;Gerisch, G
通讯作者：
Gerisch, G
影响因子：
--
作者：
Drumm, K;Lee, E;Pollock, CA
通讯作者：
Pollock, CA