Screening and identification of host factors interacting with UL14 of herpes simplex virus 1
Screening and identification of host factors interacting with UL14 of herpes simplex virus 1
复制标题
单纯疱疹病毒1型UL14互作宿主因子的筛选与鉴定
DOI：
10.1007/s00430-011-0196-z
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发表时间：
2011-04
影响因子：
5.4
通讯作者：
Wang, Shuai
中科院分区：
文献类型：
--
作者：
Li, Meili;Zheng, Chunfu;Xing, Junji;Wu, Fuqing;Wang, Shuai
单纯疱疹病毒1型（HSV-1）的UL 14蛋白在疱疹病毒家族中高度保守。然而，其在HSV-1复制周期中的确切功能却鲜为人知。本研究利用高通量酵母双杂交系统筛选与UL 14相互作用的细胞因子，得到5个候选靶点：TSC 22结构域家族蛋白3（TSC 22 D3）、RNA聚合酶II转录亚基8亚型1（MED 8）、Runt相关转录因子3（RUNX 3）、RNA聚合酶II转录亚基8亚型1（MED 8）（4）抑制蛋白β_2（ARRB 2）;（5）Cereblon（CRBN）。间接免疫荧光检测显示TSC 22 D3和MED 8均与UL 14共定位。免疫共沉淀实验表明，UL 14可以被TSC 22 D3免疫沉淀，表明UL 14与TSC 22 D3在生理条件下相互作用。总之，这项研究开辟了新的途径，描绘的功能和生理意义的UL 14在HSV-1复制周期。
The UL14 protein of herpes simplex virus type 1 (HSV-1) is highly conserved in herpesvirus family. However, its exact function during the HSV-1 replication cycle is little known. In the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with UL14, and five target candidates were yielded: (1) TSC22 domain family protein 3 (TSC22D3); (2) Mediator of RNA polymerase II transcription subunit 8 isoform 1(MED8); (3) Runt-related transcription factor 3 (RUNX3); (4) Arrestin beta-2 (ARRB2); (5) Cereblon (CRBN). Indirect immunofluorescent assay showed that both TSC22D3 and MED8 co-localized with UL14. Co-immunoprecipitation assay demonstrated that UL14 could be immunoprecipitated by TSC22D3, suggesting that UL14 interacted with TSC22D3 under physiological condition. In summary, this study opened up new avenues toward delineating the function and physiological significance of UL14 during the HSV-1 replication cycle.
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通讯作者：
Qihan, Wu
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影响因子：
5.4
作者：
Lin, Fusen;Pan, Weiwei;Zheng, Chunfu;Hu, Chang-Deng;Xing, Junji;Wang, Shuai
通讯作者：
Wang, Shuai
影响因子：
32.4
作者：
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通讯作者：
Riccardi, C