Methane fermentation of Japanese cedar wood pretreated with a white rot fungus, Ceriporiopsis subvermispora.
Methane fermentation of Japanese cedar wood pretreated with a white rot fungus, Ceriporiopsis subvermispora.
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DOI：
10.1016/j.jbiotec.2005.10.004
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发表时间：
2006-05
影响因子：
4.1
通讯作者：
R. Amirta;T. Tanabe;Takahito Watanabe;Y. Honda;M. Kuwahara;Takashi Watanabe
中科院分区：
文献类型：
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作者：
R. Amirta;T. Tanabe;Takahito Watanabe;Y. Honda;M. Kuwahara;Takashi Watanabe
用4株白腐菌Citporiopsis subvermispora ATCC 90467、CZ-3、CBS 347.63和Pleurocybella porrigens K-2855对日本雪松木材进行了甲烷发酵。这些真菌在添加和不添加麦皮的木片上培养4-8周。用污水处理厂的污泥厌氧发酵处理过的木片。枯草芽孢杆菌ATCC 90467、CZ-3和CBS 347.63在小麦麸皮存在下处理8周，木质素中β-O-4芳基醚的键减少了74-76%，从而促进了甲烷的产生。用枯草芽孢杆菌ATCC 90467进行真菌处理后，经过30天的甲烷发酵，以腐烂木材和原始木材的综纤维素含量计算，甲烷产量分别达到理论产量的35%和25%。相比之下，用这三种不含小麦麸皮的枯草芽孢杆菌菌株处理可以切断15%-26%的连接，并产生6%-9%的甲烷。对木质素分解能力较低的P.porrigens处理的木片没有明显的加速作用。结果表明，枯草芽孢杆菌具有较强的分解木质素芳基醚键的能力，在麦麸皮存在下，能促进针叶木甲烷发酵。
Methane fermentation of Japanese cedar wood was carried out after pretreatment with four strains of white rot fungi, Ceriporiopsis subvermispora ATCC 90467, CZ-3, CBS 347.63 and Pleurocybella porrigens K-2855. These fungi were cultivated on wood chip media with and without wheat bran for 4–8 weeks. The pretreated wood chip was fermented anaerobically with sludge from a sewage treatment plant. Pretreatments with C. subvermispora ATCC 90467, CZ-3 and CBS 347.63 in the presence of wheat bran for 8 weeks decreased 74–76% of β-O-4 aryl ether linkages in the lignin to accelerate production of methane. After fungal treatments with C. subvermispora ATCC 90467 and subsequent 30-days methane fermentation, the methane yield reached 35 and 25% of the theoretical yield based on the holocellulose contents of the decayed and original wood, respectively. In contrast, treatment with the three strains of C. subvermispora without wheat bran cleaved 15–26% of the linkage and produced 6–9% of methane. There were no significant accelerating effects in wood chips treated with P. porrigens which has a lower ability to decompose the lignin. Thus, it was found that C. subvermispora, with a high ability to decompose aryl ether bonds of lignin, promoted methane fermentation of softwood in the presence of wheat bran.