Functional morphologies of retinal ganglion cells in the turtle

Functional morphologies of retinal ganglion cells in the turtle
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DOI:
10.1002/cne.903500409
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发表时间:
1994-12
影响因子:
2.5
通讯作者:
C. A. Kittila;A. M. Granda
C. A. Kittila;A. M. Granda
中科院分区:
医学3区
文献类型:
--
作者:
C. A. Kittila;A. M. Granda

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采用静止和移动光源记录的方法,研究了黄斑鱼视网膜神经节细胞。反应在OFF、ON、ON - OFF类别和定向选择性之间进行了分配。细胞注射神经生物素,然后按照标准程序与亲和素-辣根过氧化物酶偶联。染色细胞的形态学分析包括测量体细胞和树突场大小、树突分层、细胞突数、树突分支和树突对称比。
Retinal ganglion cells in the turtle, Pseudemys scripta elegans, were examined by intracellular recording with a protocol of stationary and moving lights. Responses were apportioned among OFF, and ON, and ON‐OFF categories, and directional selectivity. Cells were injected with Neurobiotin, then later conjugated with avidin‐horseradish peroxidase in standard procedure. Morphological analysis of the stained cells included measurements of soma and dendritic field sizes, dendritic stratification, number of cell processes, dendritic branchings, and dendritic symmetry ratios.