Human immunodeficiency virus type 1 gp160 and gp41 binding to Candida albicans selectively enhances candidal virulence in vitro
Human immunodeficiency virus type 1 gp160 and gp41 binding to Candida albicans selectively enhances candidal virulence in vitro
复制标题
DOI：
10.1086/515231
复制
发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Würzner, R
中科院分区：
文献类型：
--
作者：
Gruber, A;Lukasser-Vogl, E;Würzner, R
此前，已有研究表明人类免疫缺陷病毒(HIV)-1囊膜蛋白gp160和gp41与白色念珠菌结合。研究了这种相互作用是否会影响念珠菌的体外毒力。HIV-1gp160或gp120对白色念珠菌的处理既没有显著改变真菌的生长，也没有显著改变磷脂酶活性。然而，用gp160处理白色念珠菌，而不用gp120处理，会导致游离和细胞结合的天冬氨酸蛋白酶的升高。此外，用gp160或gp41处理而不用gp120处理的白色念珠菌的培养上清液显示出强烈的蛋白酶活性增加。最后，用gp160或gp41和血清处理的白色念珠菌活酵母细胞被多形核白细胞吞噬的程度比用gp120和血清或单独用血清处理的白色念珠菌要小。这些发现表明，HIV-1 gp160和白色念珠菌之间的相互作用可能会促进HIV-1阳性患者中白色念珠菌的毒力。
Previously, it has been shown that human immunodeficiency virus (HIV)-1 envelope proteins gp160 and gp41 bind to Candida albicans. Whether this interaction affects candidal virulence in vitro was investigated. HIV-1 gp160 or gp120 treatment of C. albicans significantly altered neither growth nor phospholipase activity of the fungus. However, treatment of C. albicans with gp160, but not with gp120, led to an elevation of free and cell-bound aspartate proteinase. In addition, culture supernatants obtained from C. albicans treated with gp160 or gp41, but not with gp120, showed a strong increase in proteinase activity. Finally, C. albicans viable yeast cells treated with gp160 or gp41 and serum were phagocytosed by polymorphonuclear leukocytes to a lesser extent than was C. albicans treated with gp120 and serum or serum alone. These findings suggest that the interaction between HIV-1 gp160 and C. albicans may promote the virulence of C. albicans in HIV-1-positive patients.