Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometry
Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometry
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DOI：
10.1002/btpr.3311
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发表时间：
2022-10
影响因子：
2.9
通讯作者：
Sarah K. Wysor;Katja A. Hall;R. K. Marcus
中科院分区：
文献类型：
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作者：
Sarah K. Wysor;Katja A. Hall;R. K. Marcus
细胞培养基金属含量在哺乳动物细胞生长和单克隆抗体生产中至关重要。金属浓度的变化有多种来源。因此，需要在生产之前、期间和之后分析介质。此外，影响过程的不仅仅是某种金属的存在，还有它们的化学形式，即物种形成。对于第一近似，简单快速地确定金属是作为无机（游离金属）离子存在还是作为有机金属络合物（配位）的一部分是有益的。在这里，我们提出了一个简单的工作流程，涉及捕获连接的金属纤维固定相的自由离子通过电感耦合等离子体发射光谱进行定量，捕获的物种随后洗脱定量。该第一水平的物种形成（游离的与连接的）可以是关于污染物金属物种的来源和评估生物反应器过程的手段的信息。
Cell culture media metal content is critical in mammalian cell growth and monoclonal antibody productivity. The variability in metal concentrations has multiple sources of origin. As such, there is a need to analyze media before, during, and after production. Furthermore, it is not the simple presence of a given metal that can impact processes, but also their chemical form that is, speciation. To a first approximation, it is instructive to simply and quickly ascertain if the metals exist as inorganic (free metal) ions or are part of an organometallic complex (ligated). Here we present a simple workflow involving the capture of ligated metals on a fiber stationary phase with passage of the free ions to an inductively coupled plasma optical emission spectrometry for quantification; the captured species are subsequently eluted for quantification. This first level of speciation (free vs. ligated) can be informative towards sources of contaminant metal species and means to assess bioreactor processes.