Implantation of Capillary Structure Engineered by Optical Lithography Improves Hind Limb Ischemia in Mice
Implantation of Capillary Structure Engineered by Optical Lithography Improves Hind Limb Ischemia in Mice
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DOI：
10.1089/ten.tea.2009.0097
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发表时间：
2010-03-01
影响因子：
4.1
通讯作者：
Morita, Ikuo
中科院分区：
文献类型：
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作者：
Akahori, Taichi;Kobayashi, Akiko;Morita, Ikuo
我们以前报道了一种新的光学光刻技术，用于构建由内皮细胞组成的毛细血管网络。为探讨临床应用于治疗缺血性疾病的可行性，将羊膜（AM）制成支架，并植入小鼠体内，形成毛细血管结构。毛细血管网络保持在原位至少5天，并在耳瓣模型中组织学检测通过植入毛细血管的血液灌注。此外，在插入后5天，观察到血液流过植入小鼠腹部皮下组织中的毛细血管网。与对照组相比，将AM毛细血管结构植入小鼠缺血后肢显著增加了再灌注（仅AM）。术后第9天，缺血肢体的血流恢复到相应非缺血肢体的水平。治疗逆转了缺血症状，行走障碍明显改善。因此，植入离体工程化的毛细血管网络可能具有治疗缺血性疾病的潜力。
We previously reported a novel optical lithographic technique for the construction of a capillary network consisting of endothelial cells. To investigate the feasibility of clinical application in the treatment of ischemic diseases, capillary structures were formed on scaffolds made from amniotic membrane (AM) and implanted into mice. The capillary network remained in place for at least 5 days and blood perfusion through the implanted capillaries was histologically detected in an ear flap model. Moreover, blood was observed flowing through the capillary network implanted in abdominal subcutaneous tissue of mice at 5 days after insertion. Implantation of the AM capillary structure into the ischemic hind limbs of mice significantly increased reperfusion compared with controls (AM only). Blood flow was restored in the ischemic limbs to the level of corresponding non-ischemic limbs as early as 9 days after surgical implantation. The treatment reversed ischemic symptoms, and ambulatory impairment was significantly improved. Thus, the implantation of a capillary network engineered ex vivo could have therapeutic potential for ischemic diseases.