Microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection
Microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection
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DOI：
10.1016/j.vetpar.2014.07.018
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发表时间：
2014-09-15
影响因子：
2.6
通讯作者：
Machado, Marco Antonio
中科院分区：
文献类型：
--
作者：
Carvalho, Wanessa Araujo;Domingues, Robert;Machado, Marco Antonio
蜱叮咬促进炎症过程的激活，这是由牛的遗传组成和它以前的接触史的影响。已知牛磺酸和靛豆碱品种对微小扇头蜱的免疫应答发展不同。然而，进一步调查的复杂分子途径参与发展的免疫反应蜱感染牛呈现相同的遗传背景是强制性的。本工作的目的是了解由R. microplus幼虫附着在先前选择的抗性和易感动物中的牛F2群体来自Gyr（Bos indicus）x Holstein（Bos taurus）杂交。用微阵列数据分析蜱感染皮肤的RNA样品，以评估R.微小幼虫附着我们的实验设计使我们能够深入探索与R相关的免疫反应。微虫侵染避免了这些品种之间的先天差异。发现的差异表达基因揭示了网络和途径，表明脂质代谢在抵抗动物的炎症控制和蜱虫侵染损伤中起关键作用。急性期反应似乎也在易感动物中受损。这些结果提供了关于对蜱虫的早期免疫反应的新见解，并提高了使用免疫调节过程来改进和开发蜱虫控制新工具的可能性。(C)2014爱思唯尔有限公司版权所有。
Tick bites promote activation of an inflammatory process that is influenced by bovine genetic composition and its history of previous exposure. Taurine and indicine breeds are known to differ on its immune response development against Rhipicephalus microplus. Nevertheless, further investigation about the complex molecular pathways involved in the development of immune response to tick infestation in cattle presenting the same genetic background is mandatory. The aim of this work was to access the early immune response triggered by R. microplus larvae attachment in previously selected resistant and susceptible animals in a bovine F2 population derived from Gyr (Bos indicus) x Holstein (Bos taurus) crosses. Microarray data analysis of RNA samples from tick infested skin was used to evaluate the gene expression at 0, 24 and 48 h after R. microplus larvae attachment. Our experimental design allowed us to deeply explore the immune response related to R. microplus infestation avoiding the innate differences between these breeds. The differentially expressed genes found reveal networks and pathways that suggest a key role of lipid metabolism in inflammation control and impairment of tick infestation in resistant animals. Acute phase response also seems to be impaired in susceptible animals. These results provide new insights about early immune response against ticks and raise the possibility of using immunomodulation processes to improve and develop novel tools for tick control. (C) 2014 Elsevier B.V. All rights reserved.