Nucleotide‐Driven Molecular Sensing of Monkeypox Virus Through Hierarchical Self‐Assembly of 2D Hafnium Disulfide Nanoplatelets and Gold Nanospheres
Nucleotide‐Driven Molecular Sensing of Monkeypox Virus Through Hierarchical Self‐Assembly of 2D Hafnium Disulfide Nanoplatelets and Gold Nanospheres
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DOI：
10.1002/adfm.202212569
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发表时间：
2023-02
影响因子：
19
通讯作者：
Panchali Moitra;Maria Iftesum;David Skrodzki;Priyanka Paul;Elnaz Sheikh;Jennifer L. Gray;K. Dighe;Zach Sheffield;M. Gartia;D. Pan
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文献类型：
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作者：
Panchali Moitra;Maria Iftesum;David Skrodzki;Priyanka Paul;Elnaz Sheikh;Jennifer L. Gray;K. Dighe;Zach Sheffield;M. Gartia;D. Pan
液体界面促进了具有适用于分子诊断的等离子体特性的纳米级生物材料的组织。使用2D二硫化铪纳米片和零维球形金纳米颗粒的分级组装，报道了多功能材料的设计。当目标分析物存在时，纳米复合材料的自组装模式发生变化，从而改变其等离子体响应。以猴痘病毒（MPXV）为例，研究结果表明，将基因组DNA添加到纳米复合材料表面会增加金纳米颗粒之间的团聚，并降低二硫化铪纳米片之间的π堆积距离。此外，发现这种自组装纳米材料对其他病原体具有最小的交叉反应性，检测限为7.6 pg µL−1（即，3.57× 104拷贝µL−1）向MPXV。总的来说，这项研究有助于更好地了解MPXV的基因组结构，以化学设计和开发靶向核苷酸。该研究已通过紫外可见光谱，X射线衍射，扫描透射电子显微镜，表面增强拉曼显微镜和电磁模拟研究进行了验证。据了解，这是文献中第一项报告在几分钟内选择性分子检测MPXV的研究，而不使用任何高端仪器技术，如聚合酶链反应。
Liquid interfaces facilitate the organization of nanometer‐scale biomaterials with plasmonic properties suitable for molecular diagnostics. Using hierarchical assemblage of 2D hafnium disulfide nanoplatelets and zero‐dimensional spherical gold nanoparticles, the design of a multifunctional material is reported. When the target analyte is present, the nanocomposites’ self‐assembling pattern changes, altering their plasmonic response. Using monkeypox virus (MPXV) as an example, the findings reveal that adding genomic DNA to the nanocomposite surface increases the agglomeration between gold nanoparticles and decreases the π‐stacking distance between hafnium disulfide nanoplatelets. Further, this self‐assembled nanomaterial is found to have minimal cross‐reactivity toward other pathogens and a limit of detection of 7.6 pg µL−1 (i.e., 3.57 × 104 copies µL−1) toward MPXV. Overall, this study helped to gain a better understanding of the genomic organization of MPXV to chemically design and develop targeted nucleotides. The study has been validated by UV–vis spectroscopy, X‐ray diffraction, scanning transmission electron microscopy, surface‐enhanced Raman microscopy and electromagnetic simulation studies. To the best knowledge, this is the first study in literature reporting selective molecular detection of MPXV within a few minutes and without the use of any high‐end instrumental techniques like polymerase chain reactions.