Detection and Characterization of ESBL-Producing Escherichia coli From Humans and Poultry in Ghana
Detection and Characterization of ESBL-Producing Escherichia coli From Humans and Poultry in Ghana
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DOI：
10.3389/fmicb.2018.03358
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发表时间：
2019-01-15
影响因子：
5.2
通讯作者：
Eibach, Daniel
中科院分区：
文献类型：
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作者：
Falgenhauer, Linda;Imirzalioglu, Can;Eibach, Daniel
在撒哈拉以南非洲，由广谱β -内酰胺酶（ESBL）产生的大肠杆菌引起的感染发生率日益增加，这是一个令人严重关注的问题。家禽业高度工业化国家的研究表明，家禽生产-食品-消费链是潜在的传播途径。在非洲，关于人与动物界面的综合研究仍然缺失。目的：了解加纳农村人群和家禽肠道产esbl大肠杆菌的分子流行病学。方法：在6个月的时间里，收集所有在Agogo医院（加纳）住院的儿童和医院集水区内8个家禽养殖场的肉鸡的粪便样本。在选择性ESBL琼脂上筛选后，对所有分离株进行全基因组测序（WGS）。采用多位点序列分型（MLST）、ESBL基因分型和基于基因组的系统发育分析对基因组进行分析。结果：在140只肉鸡和54只儿童中，分别有41只（29%）和33只（61%）携带ESBL大肠杆菌，养殖场患病率为0% ~ 85%。在人类中未检测到优势序列型（ST）。ST10在肉鸡中最为普遍（n = 31, 69%）。ESBL基因bla(C)(Tx-M)（-15）在肉鸡（n = 43,96%）和人（n = 32,97%）中占主导地位。基于全基因组的系统发育分析显示，三个非常密切相关的肉鸡/人分离簇（10%的ESBL分离株）具有染色体和质粒介导的ESBL基因。结论：研究结果表明，加纳农村地区肠道中产生esbl的大肠杆菌的频率很高。考虑到动物和人类样本是来自同一地理位置的独立样本，因此在这两个库中传播的密切相关的ESBL分离株数量很大。因此，家禽养殖场或肉制品可能是加纳农村产生esbl细菌的重要来源，导致人类感染难以治疗。
Introduction: The increasing incidence of infections caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in sub-Saharan Africa is of serious concern. Studies from countries with a highly industrialized poultry industry suggest the poultry production-food-consumer chain as a potential transmission route. In Africa, integrated studies at this human-animal interface are still missing.Aim: To determine the molecular epidemiology of ESBL-producing E. coli from the intestinal tract of humans and poultry in rural Ghana.Methods: During a 6-month period, fecal samples from all children admitted to the Agogo Hospital (Ghana) and broilers at eight poultry farms located within the hospital catchment area were collected. After screening on selective ESBL agar, whole genome sequencing (WGS) was performed on all ESBL isolates. The genomes were analyzed using multilocus sequence typing (MLST), ESBL genotyping and genome-based phylogenetic analyses.Results: Of 140 broilers and 54 children, 41 (29%) and 33 (61%) harbored ESBL E coli, respectively, with prevalences on farms ranging between 0 and 85%. No predominant sequence type (ST) was detected among humans. ST10 was most prevalent among broilers (n = 31, 69%). The ESBL gene bla(C)(Tx-M)(-15) was predominant among broilers (n = 43, 96%) and humans (n = 32, 97%). Whole-genome-based phylogenetic analysis revealed three very closely related broiler/human isolate clusters (10% of ESBL isolates) with chromosomal and plasmid-mediated ESBL genes.Conclusion: The findings demonstrate a high frequency of intestinal ESBL-producing E coli in rural Ghana. Considering that animal and human samples are independent specimens from the same geographic location, the number of closely related ESBL isolates circulating across these two reservoirs is substantial. Hence, poultry farms or meat products might be an important source for ESBL-producing bacteria in rural Ghana leading to difficult-to-treat infections in humans.