Effect of pentagastrin on gastric mucosal histamine in dogs.
Effect of pentagastrin on gastric mucosal histamine in dogs.
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五肽胃泌素对狗胃粘膜组胺的影响。
DOI：
10.1152/ajpgi.1985.248.3.g369
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Richardson,CT
中科院分区：
文献类型：
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作者：
Redfern,JS;Thirlby,R;Feldman,M;Richardson,CT
我们研究了五肽胃泌素对患有胃瘘的狗的胃粘膜中组胺含量的影响，以确定五肽胃泌素是否动员了组胺的细胞储存。在离体犬胃粘膜上进行实验，以研究五肽胃泌素对组胺释放的影响。在体内研究中，基础组胺含量平均为0.9 nmol/mg湿重组织或18.8 nmol/mg组织蛋白。静脉注射五肽胃泌素（6或16 μ g）期间，胃粘膜组胺含量无显著差异。kg-1 . h-1）或盐水（对照），即使在五肽胃泌素输注期间来自胃瘘的酸输出显著增加超过对照。此外，五肽胃泌素（10（-5）和10（-8）M）在体外从胃粘膜释放的组胺并不比缓冲液（对照）多，而Triton X-100和佛波酯4 β-佛波醇12 β-肉豆蔻酸酯13 α-乙酸酯单独和与钙离子载体A23187组合释放的组胺显著高于对照值。从这些实验中，我们得出结论，pentagastrin没有改变组胺含量的犬胃粘膜在体内，即使酸分泌刺激最大，也没有pentagastrin释放组胺在体外。
We studied the effect of pentagastrin on histamine content of gastric mucosa obtained from dogs with gastric fistulas to determine whether pentagastrin mobilizes cellular stores of histamine. Experiments were also performed on canine gastric mucosa in vitro to investigate the effects of pentagastrin on histamine release, per se. During in vivo studies basal histamine content averaged 0.9 nmol/mg wet wt tissue or 18.8 nmol/mg tissue prot. No significant difference in gastric mucosal histamine content occurred during intravenous administration of pentagastrin (6 or 16 micrograms . kg-1 . h-1) or saline (control), even though acid output from the gastric fistula increased significantly above control during pentagastrin infusion. Moreover, pentagastrin (10(-5) and 10(-8)M) did not release more histamine from gastric mucosa in vitro than the buffer (control), whereas Triton X-100 and the phorbol ester 4 beta-phorbol 12 beta-myristate 13 alpha-acetate, alone and in combination with calcium ionophore A23187, released significant amounts of histamine above control values. From these experiments we conclude that pentagastrin did not alter histamine content of canine gastric mucosa in vivo, even though acid secretion was stimulated maximally, nor did pentagastrin release histamine in vitro.