Proteome analysis of human placentae: Pre-eclampsia versus normal pregnancy
Proteome analysis of human placentae: Pre-eclampsia versus normal pregnancy
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DOI：
10.1016/j.placenta.2006.10.005
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发表时间：
2007-07-01
期刊：
影响因子：
3.8
通讯作者：
Takeshita, T.
中科院分区：
文献类型：
--
作者：
Mine, K.;Katayama, A.;Takeshita, T.
尽管胎盘蛋白在胎儿和胎盘发育以及妊娠维持中发挥多种作用，但许多仍不充分。在本研究中，我们通过使用蛋白质组学方法全面分析了这些蛋白质。样品用盐酸胍变性，这被认为是上级常用的尿素用于本目的，并进行二维（2D）电泳（2-DE），以获得胎盘蛋白质组图谱。识别的蛋白质点（ca.在蛋白质组图谱上的蛋白质（占总数的60%）包括几种妊娠相关蛋白（PRPs）。此外，一种新的二维免疫印迹（2-DI）分析分子相关的先兆子痫揭示了三个免疫阳性斑点，似乎对应于dynactin p-50，一种蛋白质相关的细胞翻转。阳性率为dynactin p-50反应性抗体显着（P = 0.0024）高于58正常妊娠妇女在26先兆子痫的妇女。这些结果表明，dynactin p-50可能参与先兆子痫的病理生理。(C)2006爱思唯尔有限公司保留所有权利。
Although placental proteins play multiple roles in fetal and placental development and in the maintenance of pregnancy, many remain inadequately characterized. In the present study, we comprehensively analyzed these proteins by using a proteomic approach. Samples were denatured with guanidine hydrochloride, which was found to be superior to the commonly used urea for the present purpose, and subjected to 2-dimensional (2D) electrophoresis (2-DE) to obtain placental proteome maps. The identified protein spots (ca. 60% of the total) on the proteome maps included several pregnancy-related proteins (PRPs). Furthermore, a novel 2D immunoblotting (2-DI) analysis of molecules related to pre-eclampsia revealed three immunopositive spots that appeared to correspond to dynactin p-50, a protein related to cell tum-over. The rate of positivity for dynactin p-50-reactive antibodies was significantly (P = 0.0024) higher in 26 pre-eclamptic women than in 58 normally pregnant women. These results indicate that dynactin p-50 may be involved in the pathophysiology of pre-eclampsia. (C) 2006 Elsevier Ltd. All rights reserved.