Annexin VSPECT imaging of phosphatidylserine expression in patients with dementia
Annexin VSPECT imaging of phosphatidylserine expression in patients with dementia
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DOI：
10.1212/01.wnl.0000208436.75615.8c
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发表时间：
2006-04-25
期刊：
影响因子：
9.9
通讯作者：
Gilad, R
中科院分区：
文献类型：
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作者：
Lampl, Y;Lorberboym, M;Gilad, R
自最初描述以来，尚未有关于使用 Tc-99m-HYNIC-annexin V（磷脂酰丝氨酸 (PS) 表达标记物）对阿尔茨海默氏痴呆 (AD) 患者进行成像的报道。 (1) 膜联蛋白 V 是一种普遍存在的 35 kDa 内源性细胞内蛋白，可与阴离子膜磷脂 (PS) 结合。 (2)在细胞凋亡的早期阶段，在caspase-3激活后，PS立即在细胞上选择性地大量表达。然而，在轻度缺血/再灌注相关应激的条件下，已发现低至中等水平的PS，这可以通过及时再灌注和正常水平的氧来逆转。 (3) 此外，已发现 Tc-99m-HYNIC-annexin V 定位于动物模型中的缺血性损伤部位，甚至在血脑屏障功能完整的区域也是如此。 (4,5) 在本研究中，我们检查了通过 Tc-99m-HYNIC-annexin V SPECT 定义的 AD 患者大脑内的 PS 表达。我们还研究了非 AD 痴呆症患者和年龄匹配的无病健康对照者。
Since its initial description, there have been no reports on the use of Tc-99m-HYNIC-annexin V, a marker of phosphatidylserine (PS) expression, for imaging patients with Alzheimer dementia (AD). (1) Annexin V is a ubiquitous 35-kDa endogenous intracellular protein that binds to the anionic membrane phospholipid, PS. (2) PS is selectively and massively expressed on cells immediately after caspase-3 activation during the early phases of apoptosis. Under conditions of mild ischemia/reperfusion related stress, however, low to intermediate levels of PS have been found which can be reversed with timely reperfusion and normal levels of oxygen. (3) In addition, Tc-99m-HYNIC-annexin V has been found to localize to sites of ischemic injury in animal models even in regions in which the blood-brain barrier is functionally intact. (4,5)In this study, we examined PS expression within the cerebrum of patients with AD as defined by Tc-99m-HYNIC-annexin V SPECT. We also studied patients with non-AD dementia and nondiseased healthy age-matched controls.