Two types of myeloperoxidase-antineutrophil cytoplasmic autoantibodies with a high affinity and a low affinity in small vessel vasculitis.
Two types of myeloperoxidase-antineutrophil cytoplasmic autoantibodies with a high affinity and a low affinity in small vessel vasculitis.
复制标题
小血管炎中具有高亲和力和低亲和力的两种髓过氧化物酶-抗中性粒细胞胞质自身抗体。
DOI：
10.1093/rheumatology/keq087
复制
发表时间：
2009
影响因子：
3.7
通讯作者：
M. Yamada
中科院分区：
文献类型：
--
作者：
M. Yoshida;M. Sasaki;I. Nakabayashi;M. Akashi;T. Tomiyasu;N. Yoshikawa;T. Kojima;N. Ohno;M. Yamada
目的
髓过氧化物酶（MPO）-抗中性粒细胞胞浆抗体（ANCA）在显微镜下多血管炎和肾局限性血管炎中检出率很高。MPO-ANCA滴度并不总是反映在疾病活动中。我们研究了患者血清中MPO-ANCA的滴度和亲和力与血管炎活动的关系。
方法
从27例新诊断或复发的MPO-ANCA相关血管炎患者中采集血液样本。使用均匀纯化的人白细胞MPO通过直接酶联免疫吸附测定（ELISA）测定MPO-ANCA滴度。MPO-ANCA亲和力表示为IC 50，其通过竞争性抑制方法使用ELISA测定。
结果
27例患者的27份血清的MPO-ANCA亲和力可分为高亲和力型（14份）和低亲和力型（13份）。两种类型的IC 50平均值分别为0.15+/-0.06 microg/ml和0.54+/-0.15 microg/ml，差异具有统计学意义（p<0.000000684）。在按亲和力划分的两组患者之间，伯明翰血管炎活动评分（BVAS）和C反应蛋白（CRP）存在差异（分别为p<0.00093和p<0.00129）。然而，滴度差异无统计学显著性（p<0.0265）。从疾病发作到缓解或复发，亲和力保持稳定。
结论
来自MPO-ANCA相关性血管炎患者的MPO-ANCA亲和力在很大程度上被区分为高亲和力和低亲和力，而与MPO-ANCA滴度水平无关，并且可能有助于评估主要影响肾脏和肺的血管炎活动。
OBJECTIVE
Myeloperoxidase (MPO) -anti-neutrophil cytoplasmic autoantibodies (ANCAs) are detected at a high rate in microscopic polyangiitis and renal-limited vasculitis. MPO-ANCA titers are not always reflected in the disease activity. We studied the titer and affinity of MPO-ANCA in sera from patients in relation to vasculitis activity.
METHODS
Blood samples were collected from 27 newly diagnosed or relapsed patients with MPO-ANCA-associated vasculitides. The MPO-ANCA titer was determined by a direct enzyme-linked immunosorbent assay (ELISA) using homogeneously purified human MPO of leukocytes. The MPO-ANCA affinity was expressed as IC50 that was determined by a competitive inhibition method using the ELISA.
RESULTS
The MPO-ANCA affinity of 27 sera from 27 patients could be classified into a high-affinity type (14 sera) and a low-affinity type (13 sera). The mean values for IC50 in the two types were 0.15+/-0.06 microg/ml and 0.54+/-0.15 microg/ml, and the difference was statistically significant (p<0.0000000684). Between the two groups of patients divided by the affinity, there were differences in the Birmingham Vasculitis Activity Score (BVAS): and in C-reactive protein (CRP): (p<0.00093 and p<0.00129, respectively). However, the difference in titer was not statistically significant (p<0.0265). The affinity remained steady from the disease onset to remission or relapse.
CONCLUSIONS
The affinity of MPO-ANCA from patients with MPO-ANCA-associated vasculitides were largely distinguished into a high and a low affinity, irrespective of the level of MPO-ANCA titers, and may be helpful for assessment of vasculitis activity affecting mainly the kidney and the lung.
影响因子：
19.6
作者：
Erdbrugger, U.;Hellmark, T.;Nachman, P. H.
通讯作者：
Nachman, P. H.