Expression of a kinase-defective Eph-like receptor in the normal human brain
Expression of a kinase-defective Eph-like receptor in the normal human brain
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DOI：
10.1006/bbrc.1997.6812
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发表时间：
1997-06-27
影响因子：
3.1
通讯作者：
Matsui, T
中科院分区：
文献类型：
--
作者：
Matsuoka, H;Iwata, N;Matsui, T
我们已经鉴定出人类 Eph 家族蛋白 HEP 基因，位于人类染色体区域 7q33-->q35。推导的氨基酸序列具有 Eph 家族受体酪氨酸激酶的主要结构特性。然而，保守催化结构域的 ATP 结合位点中的赖氨酸和磷酸转移位点中的天冬氨酸等六个不变氨基酸被 HEP 中的其他氨基酸残基取代，因此，在 CHO-KI 细胞转染子中表达的催化结构域中没有检测到内在的酪氨酸激酶活性，尽管大多数生长因子受体的激酶缺陷型突变体已被报道为致病性受体，但其转录物在正常成人中大量表达组织。 135 kDa HEP 蛋白在人脑中的表达量与在用 HEP cDNA 表达载体转染的 CHO-K1 细胞中的表达量相同。 HEP 是对在正常人体组织中大量表达的激酶缺陷型 Eph 家族蛋白的首次描述。 (C) 1997 年学术出版社。
We have identified a human Eph-family protein, HEP, gene located in human chromosomal region 7q33-->q35. The deduced amino acid sequence shared primary structural properties of Eph-family receptor tyrosine kinases. However, six invariant amino acids such as a lysine in the ATP-binding site and an aspartic acid in the phosphotransfer site of a conserved catalytic domain were substituted with other amino acid residues in HEP, Thus, no intrinsic tyrosine kinase activity was detectable in the catalytic domain expressed in CHO-KI cell transfectants, Although most kinase-defective mutants of growth factor receptors have been reported as pathogenic receptors, its transcript was abundantly expressed in normal human adult tissues. A 135-kDa HEP protein was expressed in the human brain as much as in CHO-K1 cells transfected with a HEP cDNA expression vector. HEP is the first description of a kinase-defective Eph-family protein expressed abundantly in normal human tissues. (C) 1997 Academic Press.