Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States
Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States
复制标题
DOI：
10.3201/eid2606.200516
复制
发表时间：
2020-06-01
影响因子：
11.8
通讯作者：
Thornburg, Natalie J.
中科院分区：
文献类型：
--
作者：
Harcourt, Jennifer;Tamin, Azaibi;Thornburg, Natalie J.
武汉市暴发肺炎的病原体中国于2020年1月被鉴定为严重急性呼吸综合征冠状病毒2型。美国一名患者于2020年1月20日被华盛顿州和美国疾病控制和预防中心诊断为感染了这种病毒。我们从该患者的鼻咽和口咽标本中分离出病毒，并对病毒序列、复制特性和细胞培养取向进行了表征。我们发现，在没有胰酶的情况下，病毒在Vero-CCL81细胞和Vero E6细胞中复制到高滴度。我们还将病毒存放在两个病毒库中，使其广泛提供给公共卫生和研究社区。我们希望这种试剂的开放使用将加快医学对策的发展。
The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.