In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, an enoate reductases and a Baeyer–Villiger monooxygenase
In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, an enoate reductases and a Baeyer–Villiger monooxygenase
复制标题
由乙醇脱氢酶、烯酸还原酶和 BaeyerâVilliger 单加氧酶组成的酶氧化还原级联的体外表征
DOI：
10.1016/j.jbiotec.2014.04.008
复制
发表时间：
2014
影响因子：
4.1
通讯作者：
M. D. Mihovilovic
中科院分区：
文献类型：
--
作者：
N. Oberleitner;C. Peters;F. Rudroff;U. T. Bornscheuer;M. D. Mihovilovic
为了更深入地了解这种多步生物催化的优点和缺点，在体外研究了由醇脱氢酶、烯酸还原酶和Baeyer-Villiger单加氧酶组成的人工酶级联。研究了由不同结构基序组成的几种底物，并提供了以高产率（高达>99%）和光学纯度（高达>99%）获得官能化手性化合物的途径。因此，所提出的酶促级联的适用性被开发用于生物可再生聚酯的合成。
An artificial enzyme cascade composed of an alcohol dehydrogenase, an enoate reductase and a Baeyer–Villiger monooxygenase was investigatedin vitroto gain deeper mechanistic insights and understand the assets and drawbacks of this multi-step biocatalysis. Several substrates composed of different structural motifs were examined and provided access to functionalized chiral compounds in high yields (up to >99%) and optical purities (up to >99%). Hence, the applicability of the presented enzymatic cascade was exploited for the synthesis of biorenewable polyesters.
影响因子：
4.6
作者：
Lowe, Jennifer R.;Martello, Mark T.;Hillmyer, Marc A.
通讯作者：
Hillmyer, Marc A.