Impaired oxidation of plasma-derived fatty acids in type 2 diabetic subjects during moderate-intensity exercise
Impaired oxidation of plasma-derived fatty acids in type 2 diabetic subjects during moderate-intensity exercise
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DOI：
10.2337/diabetes.49.12.2102
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发表时间：
2000-12-01
期刊：
影响因子：
7.7
通讯作者：
van Baak, MA
中科院分区：
文献类型：
--
作者：
Blaak, EE;van Aggel-Leijssen, DPC;van Baak, MA
本研究旨在探讨60分钟中等强度自行车运动期间脂肪酸利用的不同组成部分（Vo（2 max）的50%），8例男性2型糖尿病受试者（年龄52.6 ± 3.1岁，体脂35.8 ± 1.3%）和8名男性肥胖对照受试者（年龄45.1 +/- 1.4岁，体脂34.2 +/- 1.3%）与年龄、身体成分和最大有氧能力相匹配。为了定量脂肪酸代谢的不同组分，将[U-C-13]-棕榈酸的同位素输注与间接量热法结合使用。在单独的实验中，在输注[1，2-C-13]-乙酸盐期间测定呼气中的C-13标记回收率（乙酸盐回收因子）。各组之间碳水化合物和总脂肪氧化的能量消耗没有差异。游离脂肪酸（FFA）出现率（R-a）运动后FFA的R-a升高显著低于运动前（P < 0.05），运动后FFA的R-a升高显著低于运动前（P < 0.05）2型糖尿病组与对照组比较，P < 0.05（基线与运动[40-60分钟]; 2型糖尿病11.9 +/- 0.9 vs. 19.6 +/- 2.2 μ mol.k（-1）去脂质量[FFM].min（-1）和对照组15.8 +/- 1.8 vs. 28.6 +/- 2.1 μ mol.kg.min（-1））。2型糖尿病受试者在两种条件下血浆来源的脂肪酸氧化均显著较低（P < 0.05，基线vs.运动[40-60 min]; 2型糖尿病4.2 +/- 0.5 vs. 14.1 +/- 1.9 mol.kg mol.kg（-1）FFM.min（-1）和对照组6.2 +/- 0.6 vs. 20.4 +/- 1.9 μwww.example.com（-1）FFM.min（-1）），而甘油三酯来源的脂肪酸氧化较高（P < 0.05）。据推测，这些脂肪酸利用的损害可能在骨骼肌和肝脏胰岛素抵抗的病因学中发挥作用。
The present study was intended to investigate the different components of fatty acid utilization during a 60-min period of moderate-intensity cycling exercise (50% of Vo(2max)) in eight male type 2 diabetic subjects (aged 52.6 +/- 3.1 years, body fat 35.8 +/- 1.3%) and eight male obese control subjects (aged 45.1 +/- 1.4 years, body fat 34.2 +/- 1.3%) matched for age, body composition, and maximal aerobic capacity. To quantitate the different components of fatty acid metabolism, an isotope infusion of [U-C-13]-palmitate was used in combination with indirect calorimetry. In separate experiments, the C-13 label recovery in expired air was determined during infusion of [1,2-C-13]-acetate (acetate recovery factor). There mere no differences in energy expenditure of carbohydrate and total fat oxidation between the groups. The rate of appearance (R-a) of free fatty acid (FFA) (P < 0.05) and the exercise-induced increase in R-a of FFA were significantly lower (P < 0.05) in type 2 diabetic subjects compared with control subjects (baseline vs. exercise [40-60 min]; type 2 diabetes 11.9 +/- 0.9 vs. 19.6 +/- 2.2 mu mol.k(-1) fat-free mass [FFM].min(-1) and control 15.8 +/- 1.8 vs. 28.6 +/- 2.1 mu mol.kg(-1) FFM.min(-1)). The oxidation of plasma-derived fatty acids was significantly lower in type 2 diabetic subjects during both conditions (P < 0.05, baseline vs. exercise [40-60 min]; type 2 diabetes 4.2 +/- 0.5 vs. 14.1 +/- 1.9 mol.kg(-1) FFM.min(-1) and control 6.2 +/- 0.6 vs. 20.4 +/- 1.9 mu mol.kg(-1) FFM.min(-1)), whereas the oxidation of triglyceride-derived fatty acids was higher (P < 0.05). It is hypothesized that these impairments in fatty acid utilization may play a role in the etiology of skeletal muscle and hepatic insulin resistance.