Formation of chloroform and tetrachloroethene by Sinorhizobium meliloti strain 1021
Formation of chloroform and tetrachloroethene by Sinorhizobium meliloti strain 1021
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苜蓿中华根瘤菌菌株 1021 形成氯仿和四氯乙烯
DOI：
10.1111/lam.12462
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发表时间：
2015
影响因子：
2.4
通讯作者：
Behrens
中科院分区：
文献类型：
--
作者：
Weigold;Ruecker;Jochmann;Osorio Barajas;Zwiener;Kappler;Behrens
The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacteriumSinorhizobium melilotistrain 1021 is capable of producing up to 3338·6 ± 327·8 ng l−1headspace volume of chloroform (CHCl3) and 807·8 ± 13·5 ng l−1headspace volume of tetrachloroethene (C2Cl4) within 1 h when grown in soil extract medium. Biotic VOX formation has been suggested to be linked to the activity of halogenating enzymes such as haloperoxidases. We tested if the observed VOX formation byS. melilotican be attributed to one of its chloroperoxidases (Smc01944) that is highly expressed in the presence of H2O2.However, addition of 10 mmol l−1H2O2to theS. meliloticultures decreased VOX formation by 52% for chloroform and 25% for tetrachloroethene, while viable cell numbers decreased by 23%. Interestingly,smc01944gene expression increased 450‐fold. The quantification of extracellular chlorination activity in cell suspension experiments did not provide evidence for a role ofS. melilotichloroperoxidases in the observed VOX formation. This suggests that a momentarily unknown mechanism which requires no H2O2might be responsible for the VOX formation byS. meliloti. Regardless of the underlying mechanism our results suggest that the soil bacteriumS. melilotimight be an important source of VOX in soils.Significance and Impact of the StudyVolatile organohalogen compounds (VOX) strongly influence atmospheric chemistry and Earth's climate. Besides anthropogenic emissions they are naturally produced by either abiotic or biotic pathways in various environments. Particularly in soils, microbial processes drive the natural halogen cycle but the direct link to microbial VOX formation has not been studied in detail yet. In this study we provide evidence that the common and widespread soil bacteriumSinorhizobium melilotistrain 1021 forms chloroform and tetrachloroethene. The potential contribution ofS. melilotito soil VOX release could significantly influence soil and atmospheric chemistry.
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氯仿 – 土壤空气和大气中的浓度梯度以及土壤的排放通量
DOI：
--
发表时间：
2001
期刊：
影响因子：
--
作者：
E. Hoekstra;J. Duyzer;E. Leer;U. Brinkman
通讯作者：
U. Brinkman
影响因子：
11.4
作者：
Jiang, Jie;Bauer, Iris;Kappler, Andreas
通讯作者：
Kappler, Andreas
影响因子：
4.3
作者：
A. Ruecker;P. Weigold;S. Behrens;M. Jochmann;X. L. O. Barajas;A. Kappler
通讯作者：
A. Ruecker;P. Weigold;S. Behrens;M. Jochmann;X. L. O. Barajas;A. Kappler
对用于检测卤过氧化物酶活性的一氯二甲酮测定的批判性观点。
影响因子：
3.8
作者：
C. Wagner;I. Molitor;G. König
通讯作者：
G. König
DOI：
10.1094/mpmi.2001.14.1.86
发表时间：
2001
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Renata Santos;D. Hérouart;S. Sigaud;D. Touati;A. Puppo
通讯作者：
Renata Santos;D. Hérouart;S. Sigaud;D. Touati;A. Puppo