The EphA4 Receptor Tyrosine Kinase Is Necessary for the Guidance of Nasal Retinal Ganglion Cell Axons in Vitro
The EphA4 Receptor Tyrosine Kinase Is Necessary for the Guidance of Nasal Retinal Ganglion Cell Axons in Vitro
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DOI：
10.1006/mcne.2000.0878
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发表时间：
2000-10
影响因子：
3.5
通讯作者：
J. Walkenhorst;D. Dütting;C. Handwerker;Jisen Huai;Hideaki Tanaka;U. Drescher
中科院分区：
文献类型：
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作者：
J. Walkenhorst;D. Dütting;C. Handwerker;Jisen Huai;Hideaki Tanaka;U. Drescher
视网膜顶盖投影作为地形投影研究的模型系统。过去几年的情况表明，以弗家族的成员积极参与了这一预测的建立。迄今为止的分析主要集中在肝配蛋白配体的表征上，这些配体在顶盖和视网膜中均以梯度形式表达。在这里，我们研究了视网膜神经节细胞轴突上表达的多种 EphA 受体之一 EphA4 的作用，EphA4 在鼻和颞轴突上均匀表达。我们采用了显性失活方法和使用中和单克隆抗体的方法来灭活该受体。这些体外实验的结果表明，EphA4 至关重要地参与鼻轴突的排斥引导，但不参与颞轴突的排斥引导。
The retinotectal projection serves as a model system for the study of topographic projections. It has been shown in the past few years that members of the Eph family are strongly involved in establishing this projection. The analysis so far has focused on a characterization of Ephrin ligands which are expressed in a gradient in both the tectum and the retina. Here we investigate the role of one of the multiple EphA receptors expressed on retinal ganglion cell axons, EphA4, which is uniformly expressed on nasal and temporal axons. We have adopted both a dominant negative approach and a method using neutralizing monoclonal antibodies in order to inactivate this receptor. The results of these in vitro experiments suggest that EphA4 is crucially involved in the repulsive guidance of nasal but not of temporal axons.