Variable Field Proton-Electron Double-Resonance Imaging: Application to pH mapping of aqueous samples
Variable Field Proton-Electron Double-Resonance Imaging: Application to pH mapping of aqueous samples
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DOI：
10.1016/j.jmr.2009.11.017
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发表时间：
2010-02-01
影响因子：
2.2
通讯作者：
Samouilov, Alexandre
中科院分区：
文献类型：
--
作者：
Khramtsov, Valery V.;Caia, George L.;Samouilov, Alexandre
提出了变场质子电子双共振成像（VF PEDRI）的新概念。这允许使用专门设计的顺磁探针（例如氧气或 pH 映射）进行功能映射，并具有 MRI 高质量空间分辨率和较短的采集时间。使用模型在 200 G 场 MRI 上进行的研究表明，仅使用在 140 秒内在预选 EPR 激励场获取的两张 PEDRI 图像即可提取样品的 pH 图，提供 0.1 pH 单位的 pH 分辨率和 1.25 mm 的空间分辨率。请注意，虽然使用 pH 探针演示了功能性 VF PEDRI 的概念，但它也可用于使用专门设计的 EPR 探针研究介质的其他生物学相关参数，例如氧化还原状态、氧气或谷胱甘肽的浓度。由爱思唯尔公司出版
A new concept of Variable Field Proton-Electron Double-Resonance Imaging (VF PEDRI) is proposed. This allows for functional mapping using specifically designed paramagnetic probes (e.g. oxygen or pH mapping) with MRI high quality spatial resolution and short acquisition time. Studies performed at 200 G field MRI with phantoms show that a pH map of the sample can be extracted using only two PEDRI images acquired in 140 s at pre-selected EPR excitation fields providing pH resolution of 0.1 pH units and a spatial resolution of 1.25 mm. Note that while concept of functional VF PEDRI was demonstrated using the pH probe, it can be applied for studies of other biologically relevant parameters of the medium such as redox state, concentrations of oxygen or glutathione using specifically designed EPR probes. Published by Elsevier Inc.