LOCALIZATION OF ACETYL CHOLINE RECEPTOR BY IODINE-125 LABELED ALPHA BUNGARO TOXIN BINDING AT MOUSE MOTOR END PLATES
LOCALIZATION OF ACETYL CHOLINE RECEPTOR BY IODINE-125 LABELED ALPHA BUNGARO TOXIN BINDING AT MOUSE MOTOR END PLATES
复制标题
DOI：
10.1073/pnas.71.4.1376
复制
发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
SALPETER M M
中科院分区：
文献类型：
--
作者：
FERTUCK H C;SALPETER M M
暴露的麻醉小鼠胸锁乳突肌用~（125）I标记的α-银环蛇毒素浸泡，直到所有神经诱发的肌肉收缩消除。然后通过电子显微镜放射自显影确定标记的分布。发现标记物定位在连接褶皱的顶部，即，在最靠近轴突的连接后膜上。由于α-银环蛇毒素完全消除了生理性肌肉反应，这些结果表明，活性乙酰胆碱受体占据了连接褶皱的有限区域，并不是均匀分布在整个膜上。专门的膜密度似乎与标记的区域一致。
Exposed sternomastoid muscles of anaesthetized mice were bathed in125I-labeled α-bungarotoxin until all neurally evoked muscle contractions were eliminated. The distribution of label was then determined by electron microscope autoradiography. It was found that the label was localized at the top of the junctional folds, i.e., at the postjunctional membrane nearest the axon. Since the α-bungarotoxin had fully eliminated the physiological muscle response, these results indicate that the active acetylcholine receptor occupies a limited area of the junctional folds and is not distributed uniformly throughout this membrane. Specialized membrane densities seem to coincide with the labeled regions.