Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y-enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus
Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y-enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus
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DOI：
10.1002/j.1460-2075.1996.tb00904.x
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发表时间：
1996-10-01
期刊：
影响因子：
11.4
通讯作者：
Cornelis, GR
中科院分区：
文献类型：
--
作者：
Boland, A;Sory, MP;Cornelis, GR
耶尔森氏菌Yop病毒子是一个由四个元件组成的抗宿主系统：(I)一个称为YSC的III型分泌系统；(Ii)一个旨在将细菌蛋白输送到真核靶细胞(YopB、YopD)的系统；(Iii)一个控制元件(YopN)；以及(Iv)一组细胞内输送的蛋白质，旨在解除这些细胞的武装或干扰它们的通讯(YopE、YopH等)，YopM是另一种Yop蛋白，它与凝血酶结合，因此被认为是细胞外效应因子。在这里，我们分析了来自小肠结肠炎耶尔森菌的YopM，我们想知道它是否也可以在真核细胞内传递，为了回答这个问题，我们应用Yop-CyA报告基因策略，将141或100个YopM N端残基与CyA融合的杂交瘤细胞通过重组YopM菌株输送到PU5-1.8巨噬细胞内，YopB和YopD作为转运子。当YopN缺失时，细菌感染时巨噬细胞培养上清液中的报告分子渗漏明显增加，表明YopN参与了YopM在真核细胞内传递的调控。结论：YopM转位于真核细胞内，其生理病理作用有待进一步修正或完善。
The Yersinia Yop virulon is an anti-host system made up of four elements: (i) a type III secretion system called Ysc; (ii) a system designed to deliver bacterial proteins into eukaryotic target cells (YopB, YopD); (iii) a control element (YopN); and (iv) a set of intracellularly delivered proteins designed to disarm these cells or disrupt their communications (YopE, YopH and possibly others), YopM, another Yop protein, binds thrombin and is thus presumed to act as an extracellular effector. Here, we analyzed YopM from Y.enterocolitica and we wondered whether it could also be delivered inside eukaryotic cells, To answer this question we applied the Yop-Cya reporter strategy, Hybrids made of 141 or 100 N-terminal residues of YopM fused to Cya were delivered inside PU5-1.8 macrophages by recombinant Y.enterocolitica strains, YopB and YopD were required as translocators. Leakage of the reporters into the macrophage culture supernatant during the bacterial infection increased strongly when YopN was missing, showing that YopN is involved in the control of delivery of YopM inside eukaryotic cells. YopN itself was not delivered into the macrophages, Zn conclusion, YopM is translocated inside the eukaryotic cells and its physiopathological role should be revised or completed.